Prediction of pathogenicity islands in Enterohemorrhagic Escherichia coli O157:H7 using genomic barcodes
Prediction of pathogenicity islands in Enterohemorrhagic Escherichia coli O157:H7 using genomic barcodes
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使用基因组条形码预测肠出血性大肠杆菌 O157:H7 的致病岛
DOI：
10.1016/j.febslet.2009.11.067
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发表时间：
2010-01-04
期刊：
影响因子：
3.5
通讯作者：
Xu, Ying
中科院分区：
文献类型：
--
作者：
Wang, Guoqing;Zhou, Fengfeng;Xu, Ying
肠出血性大肠杆菌（EHEC）O157：H7的基因组具有多个致病岛（PAI）的特征。已经开发了计算方法来基于在一些派与非PAI区域中的区别性G + C水平来识别PAI。我们观察到PAI可以具有与宿主染色体非常相似的G + C水平，这可能导致使用这些方法的假阴性预测。我们应用了一种新的基因组条形码方法来鉴定PAI。使用这种技术，我们已经成功地确定了已知的和新的PAI的基因组中的三个菌株的肠出血性大肠杆菌O157：H7。(C)2009年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
The genome of lethal animal pathogenic bacterium Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is characterized by the presence of multiple pathogenicity islands (PAIs). Computational methods have been developed to identify PAIs based on the distinguishing G + C levels in some PAI versus non-PAI regions. We observed that PAIs can have a very similar G + C level to that of the host chromosome, which may have led to false negative predictions using these methods. We have applied a novel method of genomic barcodes to identify PAIs. Using this technique, we have successfully identified both known and novel PAIs in the genomes of three strains of EHEC O157: H7. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.