The nature of extra-chromosomal maintenance of transforming plasmids in the filamentous basidiomycete Phanerochaete chrysosporium.
The nature of extra-chromosomal maintenance of transforming plasmids in the filamentous basidiomycete Phanerochaete chrysosporium.
复制标题
丝状担子菌Phanerochaete chrysosporium中转化质粒的染色体外维持的性质。
DOI：
10.1007/bf00336850
复制
发表时间：
1992
期刊：
影响因子：
2.5
通讯作者：
Reddy,CA
中科院分区：
文献类型：
--
作者：
Randall,TA;Reddy,CA
对转化质粒p12-6在黄孢原毛平革菌中的染色体外维持性质进行了研究。我们的结果表明，该质粒作为一个更大的内源质粒(命名为PME)OFP的一部分，在染色体外保留在真菌转化子中。黄孢子菌。利用p12-6真菌转化子的总DNA，在A−E.colistrain中回收了p12-6和一个较大的质粒p511，而在A+E.colistrain中仅回收了p12-6。结果还表明，胞嘧啶甲基化系统对p12-6和p511的菌株依赖的回收率没有明显的影响。从真菌转化子的总DNA中提取大肠杆菌。
The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 inPhanerochaete chrysosporiumwas studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) ofP. chrysosporium. Using the total DNA of p12-6 fungal transformants, p12-6, as well as a larger plasmid, p511, were recovered inrecA−E. colistrains while only p12-6 was recovered inrecA+E. colistrains. The results also showed that the cytosine methylation system has no apparent effect on the strain-dependent recovery of p12-6 and p511 inE. colifrom the total DNA of fungal transformants.