Reduced amount of olfactory receptor neurons in the rat model of depression
Reduced amount of olfactory receptor neurons in the rat model of depression
复制标题
抑郁症大鼠模型中嗅觉受体神经元数量减少
DOI：
10.1016/j.neulet.2015.07.007
复制
发表时间：
2015-08-31
影响因子：
2.5
通讯作者：
Li, Zhiwei
中科院分区：
文献类型：
--
作者：
Li, Qianlu;Yang, Deyu;Li, Zhiwei
据报道，抑郁症患者的嗅觉灵敏度降低。但其病理机制尚不清楚。在重度抑郁症（MDD）中，嗅球（OB）体积减少和海马神经发生减少已被阐明。然而，尚未报道嗅上皮（OE）的变化，这可能导致MDD的嗅觉缺陷。在这种情况下，我们研究了厚度的OE在慢性不可预测的轻度应激（CASES）大鼠模型的抑郁症使用苏木精和伊红（HE）染色。同时，应用免疫组织化学方法检测嗅鞘中基底细胞（p75NGFR标记）、未成熟嗅觉受体神经元（GAP 43标记）和成熟嗅觉受体神经元（OMP标记）的变化。结果表明，OES大鼠OE的厚度、基底细胞数、未成熟的ORN和成熟的ORN均明显减少。这些结果表明，减少数量的ORN可能会导致OE萎缩在BLOSS大鼠和OE的异常可能是导致嗅觉敏感性降低的MDD的部分原因。(C)2015爱思唯尔爱尔兰有限公司版权所有。
Reduced olfactory sensitivity has been reported in depressive disorder. However, the pathological mechanism is still unclear. The reduced olfactory bulb (OB) volume and reduced hippocampal neurogenesis has been unraveled in major depressive disorder (MDD). However, changes in olfactory epithelium (OE) have not been reported, which might contribute to olfactory deficits in MDD. In the context, we investigated the thickness of OE in a chronic unpredictable mild stress (CUMS) rat model of depression using hematoxylin and eosin (HE) staining. Simultaneously, the basal cells (labeled by nerve growth factor receptor (p75NGFR)), immature olfactory receptor neurons (ORNs) (marked by growth-associated protein 43 (GAP43)) and mature ORNs (labeled by olfactory marker protein (OMP)) in OE were detected by immunohistochemistiy. The results showed that the thickness of OE, the number of basal cells, immature ORNs as well as mature ORNs decreased dramatically in the OE of CUMS rats. Those findings indicate that the reduced number of ORNs might induce OE atrophy in CUMS rats and the abnormalities of the OE may be partially responsible for the reduced olfactory sensitivity in MDD. (C) 2015 Elsevier Ireland Ltd. All rights reserved.