High-resolution hyperpolarized in vivo metabolic 13C spectroscopy at low magnetic field (48.7 mT) following murine tail-vein injection
High-resolution hyperpolarized in vivo metabolic 13C spectroscopy at low magnetic field (48.7 mT) following murine tail-vein injection
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DOI：
10.1016/j.jmr.2017.06.009
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发表时间：
2017-08-01
影响因子：
2.2
通讯作者：
Chekmenev, Eduard Y.
中科院分区：
文献类型：
--
作者：
Coffey, Aaron M.;Feldman, Matthew A.;Chekmenev, Eduard Y.
使用临床规模的Biplanar（80 cm-ap）48.7 mt pertenent磁体，在体外和体内报告了高极化琥珀酸酯1-C-13-2,3-D（2）的高分辨率C-13 NMR光谱。每2 s每2 s的躯干上，在0.52 MHz的谐振频率下记录了非定位的C-13 NMR光谱。在体外（注射器中的2 mL）和体内（在小鼠躯干上）记录了与3 Hz（相似于6 ppm）的线宽的超极化C-13 NMR信号。在小动物MRI扫描仪中以48.7吨获得的C-13 NMR光谱的全宽度（FWHM）的全宽度在48.7吨中获得的比较表明，与48.7吨相比，可在48.7吨tin tin Vitro的C-13谐振线宽度相似。与在高场（> = 3 T）研究中观察到的HP对比剂相比，体内的C-13超极化琥珀酸盐1-C-13共振线路至少在48.7吨时的数量级窄一个数量级。所证明的高分辨率C -13体内光谱法可能对使用具有足够大的C -13化学移位差异的HP对比剂来监测HP剂摄取或检测代谢的高敏化光谱研究很有用。 （c）2017 Elsevier Inc.保留所有权利。
High-resolution C-13 NMR spectroscopy of hyperpolarized succinate-1-C-13-2,3-d(2) is reported in vitro and in vivo using a clinical-scale, biplanar (80 cm-gap) 48.7 mT permanent magnet with a high homogeneity magnetic field. Non-localized C-13 NMR spectra were recorded at 0.52 MHz resonance frequency over the torso of a tumor-bearing mouse every 2 s. Hyperpolarized C-13 NMR signals with linewidths of similar to 3 Hz (corresponding to similar to 6 ppm) were recorded in vitro (2 mL in a syringe) and in vivo (over a mouse torso). Comparison of the full width at half maximum (FWHM) for C-13 NMR spectra acquired at 48.7 mT and at 4.7 T in a small-animal MRI scanner demonstrates a factor of similar to 12 improvement for the C-13 resonance linewidth attainable at 48.7 mT compared to that at 4.7 Tin vitro. C-13 hyperpolarized succinate-1-C-13 resonance linewidths in vivo are at least one order of magnitude narrower at 48.7 mT compared to those observed in high-field (>= 3 T) studies employing HP contrast agents. The demonstrated high -resolution C-13 in vivo spectroscopy could be useful for high -sensitivity spectroscopic studies involving monitoring HP agent uptake or detecting metabolism using HP contrast agents with sufficiently large C-13 chemical shift differences. (C) 2017 Elsevier Inc. All rights reserved.