Incorporation and remodeling of phosphatidylethanolamine containing short acyl residues in yeast
Incorporation and remodeling of phosphatidylethanolamine containing short acyl residues in yeast
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DOI：
10.1016/j.bbalip.2010.02.008
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发表时间：
2010-06-01
影响因子：
4.8
通讯作者：
Ohta, Akinori
中科院分区：
文献类型：
--
作者：
Deng, Lan;Fukuda, Ryouichi;Ohta, Akinori
磷脂酰乙醇胺（PE）是酵母（Saccharomyces cerevisiae）必需的磷脂之一。我们之前已经证明，当PE合成受到抑制时，内源性PE合成可控的酵母菌株在含有癸烷基残基（diC10PE）的PE存在下生长。在这项研究中，我们研究了diC10PE的命运，它在酵母中的摄取和重塑。编码Lem3p/Ros3p或p型atp酶Dnf1p和Dnf2p的基因缺失，会损害突变体在含有diC10PE的培养基中的生长，这表明这些蛋白参与了diC10PE的摄取。电喷雾电离串联质谱法分析了氘标记的diC10PE的代谢，发现它可以快速转化为含有C16或C18酰基残基的氘标记pe。还检测到可能含有癸酸和C16或C18脂肪酸作为酰基残基的中间pe。此外，在diC10PE的存在下，大量的癸酸被释放到培养基中。这些结果表明diC10PE被重塑成具有更长的酰基残基的pe，并被用作膜组分。ALE1和SLC1缺失突变体在diC10PE的重构中存在缺陷，其产物能够酰基转移到溶磷脂的sn-2位置，这表明它们参与了在diC10PE的重构反应中将酰基残基引入溶血磷脂酰乙醇胺的sn-2位置。我们的研究结果还表明存在一种机制来维持酵母中PE酰基残基的生理长度。(C) 2010 Elsevier B.V.版权所有
Phosphatidylethanolamine (PE) is one of the essential phospholipids in the yeast Saccharomyces cerevisiae. We have previously shown that a yeast strain, the endogenous PE synthesis of which was controllable, grew in the presence of PE containing decanoyl residues (diC10PE) when PE synthesis was repressed. In this study, we investigated the fate of diC10PE, its uptake and remodeling in yeast. Deletion of the genes encoding Lem3p/Ros3p or P-type ATPases, Dnf1p and Dnf2p, impaired the growth of the mutants in the medium containing diC10PE, suggesting the involvement of these proteins in the uptake of diC10PE. Analysis of the metabolism of deuterium-labeled diC10PE by electrospray ionization tandem mass spectrometry revealed that it was rapidly converted to deuterium-labeled PEs containing C16 or C18 acyl residues. The probable intermediate PEs that contained decanoic acid and C16 or C18 fatty acids as acyl residues were also detected. In addition, a substantial amount of decanoic acid was released into the culture medium during growth in the presence of diC10PE. These results imply that diC10PE was remodeled to PEs with longer acyl residues and used as membrane components. Defects in the remodeling of diC10PE in the deletion mutants of ALE1 and SLC1, products of which were capable of acyl-transfer to the sn-2 position of lyso-phospholipids, suggested their involvement in the introduction of acyl residues to the sn-2 position of lysophosphatidylethanolamine in the remodeling reaction of diC10PE. Our results also suggest the presence of a mechanism to maintain the physiological length of PE acyl residues in yeast. (C) 2010 Elsevier B.V. All rights reserved.