RAPID DIAGNOSIS OF CITRUS TRISTEZA VIRUS-INFECTIONS BY SODIUM DODECYL SULFATE IMMUNODIFFUSION PROCEDURES
RAPID DIAGNOSIS OF CITRUS TRISTEZA VIRUS-INFECTIONS BY SODIUM DODECYL SULFATE IMMUNODIFFUSION PROCEDURES
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DOI：
10.1094/phyto-69-88
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发表时间：
1979-01-01
期刊：
影响因子：
3.2
通讯作者：
PURCIFULL, DE
中科院分区：
文献类型：
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作者：
GARNSEY, SM;GONSALVES, D;PURCIFULL, DE
通过对兔子进行趾垫注射，有效产生了针对十二烷基硫酸钠 (SDS) 降解的柑橘 tristeza 病毒 (CTV) 外壳蛋白的抗血清。该血清在 SDS 琼脂凝胶双扩散测试中与感染了生物学上不同的 CTV 分离株的柑橘宿主提取物发生反应。该血清不会与健康柑橘提取物或感染其他病毒的柑橘提取物发生反应。纯化的CTV可以在低至1-2μg/ml的浓度下被检测到。在大多数含有韧皮部的营养组织中都发现了可检测到的 CTV 量，但在幼枝树皮中始终发现了最高滴度。病毒滴度在年轻组织中最高，并随着组织的成熟而下降，通常在温暖条件下迅速下降。通过用 6% 聚乙二醇 6000 沉淀并离心，可以从水提取物中浓缩病毒。组织可以冷冻、冻干或风干保存以供测试。对 120 棵田间树木进行 SDS 免疫扩散试验获得的结果与柑橘“墨西哥”石灰指示剂嫁接接种获得的索引结果密切相关。 The SDS-immunodiffusion procedure provides a simple, rapid approach to CTV identification applicable to both research and practical needs.
An antiserum to sodium dodecyl sulfate (SDS)-degraded citrus tristeza virus (CTV) coat protein was efficiently produced by toe-pad injection of a rabbit. This serum reacted in SDS agar gel double-diffusion tests to extracts of citrus hosts infected with biologically different CTV isolates. The serum did not react to extracts of healthy citrus or extracts of citrus infected with other viruses. Purified CTV could be detected at concentrations as low as 1-2 .mu.g/ml. Detectable amounts of CTV were found in most phloem-containing vegetative tissues, but the highest titer was consistently found in young shoot bark. Virus titer was highest in young tissues and declined, often quite rapidly under warm conditions, as tissues matured. Virus could be concentrated from aqueous extracts by precipitation with 6% polyethylene glycol 6000 and centrifugation. Tissue could be stored frozen, lyophilized, or air dried for testing. Results obtained from SDS-immunodiffusion tests of 120 field trees correlated well with indexing results obtained from graft inoculation of Citrus aurantifolia ''Mexican'' lime indicators. The SDS-immunodiffusion procedure provides a simple, rapid approach to CTV identification applicable to both research and practical needs.