Changes of antibiotic resistance genes and gut microbiota after the ingestion of goat milk.
Changes of antibiotic resistance genes and gut microbiota after the ingestion of goat milk.
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DOI：
10.3168/jds.2021-21325
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发表时间：
2022-03
影响因子：
3.5
通讯作者：
Yufang Liu;Fuxin Zhang
中科院分区：
文献类型：
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作者：
Yufang Liu;Fuxin Zhang
抗生素抗性基因作为新出现的污染物，已通过食物链成为对公众健康的严重挑战。由于肠道微生物群的丰富性和多样性，人类和动物的肠道是抗生素抗性基因发展和传播的重要库。在本研究中，我们评估了羊奶对抗生素抗性基因和肠道微生物群落（尤其是致病菌）多样性和丰度的影响。使用雄性小鼠，两组各 12 只：对照组接受无菌蒸馏水，治疗组接受羊奶，并使用宏基因组分析比较这些组的肠道微生物群和抗生素抗性基因。结果显示，摄入羊奶降低了小鼠肠道中抗生素抗性基因的多样性和丰度。干预后微生物总基因中氟喹诺酮类、肽类、大环内酯类、β-内酰胺类耐药基因的相对丰度显着下降。羊奶摄入量还显着减少了病原菌的丰度，如螺栓梭状芽胞杆菌、共生梭状芽胞杆菌、灰螺杆菌和胆螺杆菌。因此，摄入羊奶可能会降低抗生素抗性基因向肠道致病菌转移的潜力。此外，在对照组中，具有多重耐药机制的细菌约占总微生物群落的4.5%，而在羊奶组中未检测到，表明羊奶摄入完全抑制了细菌。这项研究强调了羊奶对肠道抗生素抗性组和微生物群落的影响，为进一步研究羊奶的功能提供了新的见解。
Antibiotic resistance genes, as newly emerging contaminants, have become a serious challenge to public health through the food chain. The gut of humans and animals is an important reservoir for the development and dissemination of antibiotic resistance genes because of the great abundance and diversity of intestinal microbiota. In the present study, we evaluated the influence of goat milk on the diversity and abundance of antibiotic resistance genes and gut microbial communities, especially pathogenic bacteria. Male mice were used, 12 for each of the 2 groups: a control group that received sterile distilled water and a treated group that received goat milk, and gut microbiota and antibiotic resistance genes were compared in these groups using metagenomic analysis. The results revealed that ingestion of goat milk decreased the diversity and abundance of antibiotic resistance genes in the mice gut. The relative abundance of fluoroquinolone, peptide, macrolide, and β-lactam resistance genes in the total microbial genes significantly decreased after the intervention. Goat milk intake also significantly reduced the abundance of pathogenic bacteria, such as Clostridium bolteae, Clostridium symbiosum, Helicobacter cinaedi, and Helicobacter bilis. Therefore, goat milk intake might decrease the transfer potential of antibiotic resistance gene to pathogenic bacteria in the gut. In addition, bacteria with multiple resistance mechanisms accounted for approximately 4.5% of total microbial communities in the control group, whereas it was not detectable in the goat milk group, indicating the total inhibition by goat milk intake. This study highlights the influence of goat milk on antibiotic resistome and microbial communities in the gut, and provides a new insight into the function of goat milk for further study.