Preconditioning one myocardial region does not neccessarily precondition the whole rabbit heart.
Preconditioning one myocardial region does not neccessarily precondition the whole rabbit heart.
复制标题
预处理一个心肌区域不一定预处理整个兔心脏。
DOI：
10.1007/s395-002-8385-3
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发表时间：
2002
影响因子：
9.5
通讯作者：
Downey,JamesM
中科院分区：
文献类型：
--
作者：
Nakano,Atsushi;Heusch,Gerd;Cohen,MichaelV;Downey,JamesM
以往的研究表明，预处理（PC）的一个特定的心肌区域，不仅诱发了局部心脏保护作用，但也使远程心肌梗死抵抗。在本研究中，我们设计了一种方法来测试远程PC在兔子，这是不可能结扎两个单独的冠状动脉分支在同一heart.In situ心脏受到PC与两个周期的5分钟的区域缺血/5分钟的再灌注。按照这种体内PC方案，取出心脏并在Langendorff装置上用晶体缓冲液灌注。然后，他们经历了30分钟的全脑缺血，整个左心室处于危险状态，随后再灌注2小时。在实验结束时，先前经受体内PC方案（预处理区域）的心肌被鉴定为在左冠状动脉的圈套分支的再闭塞之后将荧光颗粒注射到主动脉根中之后没有荧光的组织。氯化三苯基四氮唑染色鉴定心肌缺血。仅在预处理区域中观察到组织挽救，其中13.2 ± 3.6%的心肌梗死，而在剩余的非预处理左心室组织中为44.6 ± 1.3%（p < 0.05，n = 6）。在假手术心脏（圈套器但无PC）中，圈套器血管灌注区域和其余左心室心肌的梗死相似（49.2 ± 6.5% vs. 43.7 ± 3.7%，n = 5）。因此，一个心肌区域的PC不一定赋予PC保护心脏的所有区域。由于远程PC不能在兔子中证明，这种现象可能是物种或协议特定的，不应该假设在人类中发生。
A previous study in dogs indicated that preconditioning (PC) of a specific myocardial region not only evoked a local cardioprotective effect but also rendered remote myocardium resistant to infarction. In the present study we devised a method to test for remote PC in the rabbit which it is not possible to ligate two separate coronary branches on the same heart.In situhearts were subjected to PC with two cycles of 5-min regional ischemia/5-min reperfusion. Following thisin vivoPC protocol, the hearts were removed and perfused on a Langendorff apparatus with crystalloid buffer. They then underwent 30 min of global ischemia with the entire left ventricle at risk followed by 2 h of reperfusion. At the end of the experiment the myocardium previously subjected to thein vivoPC protocol (preconditioned region) was identified as the tissue without fluorescence after fluorescent particles had been injected into the aortic root following reocclusion of the snared branch of the left coronary artery. Infarcted myocardium was identified by triphenyltetrazolium chloride staining. Tissue salvage was observed only in the preconditioned region where 13.2 ± 3.6 % of the myocardium infarcted as opposed to 44.6 ± 1.3 % in the remaining non-preconditioned left ventricular tissue (p < 0.05, n = 6). In sham-operated hearts (snare but no PC), infarction was similar in both the snared vessel's perfusion territory and the rest of the left ventricular myocardium (49.2 ± 6.5 % vs. 43.7 ± 3.7 %, n = 5). Hence PC of one myocardial region does not necessarily confer PC protection to all regions of the heart. Because remote PC could not be demonstrated in rabbits, this phenomenon may be species or protocol-specific, and should not be assumed to occur in man.