A MAMMALIAN MITOCHONDRIAL RNA PROCESSING ACTIVITY CONTAINS NUCLEUS-ENCODED RNA
A MAMMALIAN MITOCHONDRIAL RNA PROCESSING ACTIVITY CONTAINS NUCLEUS-ENCODED RNA
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DOI：
10.1126/science.2434997
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发表时间：
1987-03-06
期刊：
影响因子：
56.9
通讯作者：
CLAYTON, DA
中科院分区：
文献类型：
--
作者：
CHANG, DD;CLAYTON, DA
核糖核酸酶线粒体 RNA 加工是一种参与哺乳动物线粒体引物 RNA 代谢的位点特异性核糖核酸内切酶，其活性需要 RNA 成分。基于互补寡核苷酸的共纯化和选择性失活，线粒体基因组中未编码的 135 核苷酸 RNA 种类被鉴定为核糖核酸内切酶的 RNA 部分。这一发现意味着细胞核编码的 RNA（细胞器 DNA 复制所必需的）被转运至线粒体基质。
Ribonuclease mitochondrial RNA processing, a site-specific endoribonuclease invlolved in primer RNA metabolism in mammalian mitochondria, requires an RNA component for its activity. On the basis of copurification and selective inactivation with complementary oligonucleotides, a 135-nucleotide RNA species, not encoded in the mitochondrial genome, is identified as the RNA moiety of the endoribonuclease. This finding implies transport of a nucleus-encoded RNA, essential for organelle DNA replication, to the mitochondrial matrix.