Genetic (co)variation of vaccine injuries and innate resistance to furunculosis (Aeromonas salmonicida) and infectious salmon anaemia (ISA) in Atlantic salmon (Salmo salar)
Genetic (co)variation of vaccine injuries and innate resistance to furunculosis (Aeromonas salmonicida) and infectious salmon anaemia (ISA) in Atlantic salmon (Salmo salar)
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DOI：
10.1016/j.aquaculture.2008.10.028
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发表时间：
2009-02-01
期刊：
影响因子：
4.5
通讯作者：
Storset, Arne
中科院分区：
文献类型：
--
作者：
Gjerde, Bjarne;Evensen, Oystein;Storset, Arne
接种疫苗的副作用，被视为内脏和壁层腹膜的粘附和变色（黑色素），在收获时对大西洋鲑鱼的2年级纯种个体进行主观评分;即，对60头公马和120头公马的1664头后代（1995年级）和32头公马和62头公马的1282头后代（1997年级）进行了研究。1995年级的个体来自单一的扇形菌株，仅在一个农场的网笼中饲养，而1997年级的个体代表四个养殖菌株和两个野生河菌株，并在两个不同的农场的每个网笼中饲养。在收获季节。雄性的粘附力和黑色素评分显著高于雌性，而养殖品系的粘附力和黑色素评分等于或低于野生品系。两个年级的估计遗传力分别为0.19 +/- 0.03（责任量表上的0.22 +/- 0.04）和0.18 +/- 0.03（责任量表上的0.31 +/- 0.06），性状之间的遗传相关性为0.71 +/- 0.08。体重（遗传率0.27 +/- 0.04）与粘附或黑色素评分之间的遗传相关性为-0.45 +/- 0.10和-0.27 +/- 0.11，因此反映了体重减轻与疫苗病变增加之间的相关性。最有可能的是，这些相关性部分是由疫苗病变对生长速率的负面非遗传效应引起的，而不是由无法从现有数据中获得的真正的遗传相关性引起的。通过使用杀鲑气单胞菌感染的大肠杆菌或通过腹膜内注射传染性鲑鱼贫血（ISAV），对相同公畜和母畜的未接种全同胞和半同胞二龄鲑前后代的另外两个样本进行了攻毒。挑战测试中生存率的遗传力估计值为0.31 +/- 0.03（责任量表为0.47 +/- 0.05），对于疖病为0.26 +/- 0.03（责任量表为0.40 +/- 0.04）。在攻毒试验期间，粘附或黑色素评分与存活率的遗传相关性均较低，与零无显著差异，因此表明疫苗病变与对呋喃丹和伊萨的抗性之间无遗传相关性。因此，疫苗病变最有可能是由免疫系统的其他成分引起的，而不是由观察到的对呋喃唑酮和伊萨的先天抗性中的遗传变异引起的。(c)2008 Elsevier B. V.保留所有权利。
Side effects from vaccination, seen as adhesion and discoloration (melanin) of the visceral and parietal peritoneum, were Subjectively scored at harvest on pedigreed individuals of two year-classes of Atlantic salmon; i.e., on 1664 offspring of 60 sires and 120 darns (year-class 1995) and 1282 offspring of 32 sires and 62 darns (year-class 1997). The 1995 year-class individuals were from a single fanned strain and were reared in a net-cage at one farm only, while the 1997 year-class individuals represented four farmed strains and two wild river strains and were reared in a net-cage at each of two different farms. At harvest. the males had significantly higher adhesion and melanin score than the females, and the farmed strains equal or lower adhesion and melanin score than the wild strains. The estimated heritability across the two year-classes was 0.19 +/- 0.03 for adhesion score (0.22 +/- 0.04 on the liability scale) and 0.18 +/- 0.03 for melanin score (0.31 +/- 0.06 on the liability scale) and the genetic correlation between the traits 0.71 +/- 0.08. The genetic correlation between body weight (heritability 0.27 +/- 0.04) and adhesion or melanin score was -0.45 +/- 0.10 and -0.27 +/- 0.11, thus reflecting an association between reduced body weight and increased vaccine lesions. Most likely these correlations were partly caused by negative non-genetic effects of vaccine lesions on growth rate, rather than by a true genetic correlation which cannot be obtained from the present data. Two additional samples of non-vaccinated full- and half-sib pre-smolt offspring of the same sires and dams were challenged either by using coliabitants infected with Aeromonas salmonicida or by intraperitoneal injection with infectious salmon anaemia (ISAV). Heritability estimates of survival in the challenge tests were 0.31 +/- 0.03 (0.47 +/- 0.05 on the liability scale) for furunculosis and 0.26 +/- 0.03 (0.40 +/- 0.04 on the liability scale) for ISAV. The genetic correlation of adhesion or melanin score With Survival during the challenge tests was all low and not significantly different from zero, thus revealing no genetic association between vaccine lesions and resistance to furunculosis and ISA. Therefore vaccine lesions are most likely caused by other components of the immune systems than those responsible for the observed genetic variation in the innate resistance to furunculosis and ISA. (c) 2008 Elsevier B.V. All rights reserved.