Natural Variation Underlies Differences in ETHYLENE RESPONSE FACTOR17 Activity in Fruit Peel Degreening
Natural Variation Underlies Differences in ETHYLENE RESPONSE FACTOR17 Activity in Fruit Peel Degreening
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DOI：
10.1104/pp.17.01320
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发表时间：
2018-03-01
期刊：
影响因子：
7.4
通讯作者：
Han, Zhenhai
中科院分区：
文献类型：
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作者：
Han, Zhenyun;Hu, Yanan;Han, Zhenhai
通过自然或人类的选择，许多肉质水果已经进化出生动的外部或内部颜色，通常在成熟过程中发展。这种颜色的发育变化与色素的生物合成以及通过叶绿素降解的退化有关。在这里，我们证明了基因乙烯反应因子17（ERF17）编码区的自然变异有助于苹果（Malus domestica）果皮去角质。具体而言，ERF17突变体等位基因与不同的丝氨酸（Ser）重复插入在编码区表现出增强的转录调控活性，在双荧光素酶报告分析时，更多的Ser重复。值得注意的是，表面等离子体共振分析表明，丝氨酸重复的数量影响ERF17的叶绿素降解相关基因的启动子序列的结合活性。此外，ERF17在万年青苹果中的过表达改变了叶绿素的积累。此外，我们证明了自苹果树种植起源以来，ERF 17一直在接受选择。综上所述，这些结果揭示了等位基因变异的一个重要的果实品质性状和与苹果驯化相关的分子遗传机制。
Through natural or human selection, many fleshy fruits have evolved vivid external or internal coloration, which often develops during ripening. Such developmental changes in color are associated with the biosynthesis of pigments as well as with degreening through chlorophyll degradation. Here, we demonstrated that natural variation in the coding region of the gene ETHYLENE RESPONSE FACTOR17 (ERF17) contributes to apple (Malus domestica) fruit peel degreening. Specifically, ERF17 mutant alleles with different serine (Ser) repeat insertions in the coding region exhibited enhanced transcriptional regulation activity in a dual-luciferase reporter assay when more Ser repeats were present. Notably, surface plasmon resonance analysis showed that the number of Ser repeats affected the binding activity of ERF17 to the promoter sequences of chlorophyll degradation-related genes. In addition, overexpression of ERF17 in evergreen apples altered the accumulation of chlorophyll. Furthermore, we demonstrated that ERF17 has been under selection since the origin of apple tree cultivation. Taken together, these results reveal allelic variation underlying an important fruit quality trait and a molecular genetic mechanism associated with apple domestication.