EVALUATION OF THE PENTOSE-PHOSPHATE PATHWAY FROM 14CO2 DATA - FALLIBILITY OF A CLASSIC EQUATION WHEN APPLIED TO NONHOMOGENEOUS TISSUES
EVALUATION OF THE PENTOSE-PHOSPHATE PATHWAY FROM 14CO2 DATA - FALLIBILITY OF A CLASSIC EQUATION WHEN APPLIED TO NONHOMOGENEOUS TISSUES
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DOI：
10.1042/bj2720127
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发表时间：
1990-11-15
影响因子：
4.1
通讯作者：
LARRABEE, MG
中科院分区：
文献类型：
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作者：
LARRABEE, MG
经常使用 14CO2 数据估计戊糖磷酸途径代谢的葡萄糖分数的经典方程，通过仔细考虑所涉及的假设及其适用条件，可以更简单地重新推导。该方程对于非均质组织来说是不可靠的，具体取决于磷酸丙糖转化为二氧化碳的比例。公式如下： ([1]CO2/G-[6]CO2/G)/(1-[6]CO2/G) = 3Fmet./(1 + 2Fmet.) 其中，[1]CO2 和 [6]CO2 分别是葡萄糖的碳 1 和碳 6 对 CO2 的输出速率，G 是葡萄糖摄取速率和 Fmet。是通过磷酸戊糖途径代谢为 CO2 和磷酸三糖的葡萄糖分数，允许回收该途径产生的适当分数的 6-磷酸果糖。该分析说明了在应用于非均质组织之前适当测试任何假设均质的方程的重要性。
A classic equation that has frequently been used to estimate the fraction of glucose metabolized by the pentose phosphate pathway, using 14CO2 data, is more simply re-derived with careful consideration of the assumptions involved and the conditions under which it is applicable. The equation is shown to be unreliable for non-homogeneous tissues, depending on the fraction of triose phosphate converted to CO2. The formula in question is as follows: ([1]CO2/G-[6]CO2/G)/(1-[6]CO2/G) = 3Fmet./(1 + 2Fmet.) where [1]CO2 and [6]CO2 are output rates of carbons 1 and 6 of glucose respectively to CO2, G is the rate of glucose uptake and Fmet. is the fraction of the glucose that is metabolized to CO2 and triose phosphate by the pentose phosphate pathway, allowing for recycling of an appropriate fraction of the fructose-6-phosphate produced by the pathway. This analysis illustrates the importance of suitably testing any equation that assumes homogeneity before application to non-homogeneous tissues.