Characterization of RAT, an autolysis regulator in Staphylococcus aureus
Characterization of RAT, an autolysis regulator in Staphylococcus aureus
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DOI：
10.1046/j.1365-2958.2003.03503.x
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发表时间：
2003-06-01
影响因子：
3.6
通讯作者：
Cheung, AL
中科院分区：
文献类型：
--
作者：
Ingavale, SS;Van Wamel, W;Cheung, AL
在试图确定参与金黄色葡萄球菌中cap 5基因调控的遗传位点时，我们分离出了一种转座子突变体，该突变体表现出生长缺陷，增强了自溶作用，并增加了对Triton X-100和青霉素的敏感性，部分原因是胞壁蛋白水解酶活性增加。对转座子插入位点侧翼的染色体序列的分析表明，突变体中被破坏的基因编码147个氨基酸的开放阅读框。我们把这个基因命名为老鼠，代表自溶活性的调节因子。序列分析表明，大鼠是同源的马尔和，在较小程度上，SarA蛋白家族。大鼠中的突变导致已知的自溶调节剂lytSR、lrgAB和arlRS的表达降低。凝胶位移研究表明，大鼠结合的lytRS和arlRS启动子，从而确认大鼠作为DNA结合蛋白，这些已知的阻遏物的自溶活性。正如预期的那样，大鼠似乎是自溶素基因（包括lytM和lytN）的负调节因子。这些数据表明，大鼠基因产物是S.金黄色。
In trying to identify genetic loci involved in the regulation of cap5 genes in Staphylococcus aureus , we isolated a transposon mutant that exhibited a growth defect, enhanced autolysis and increased sensitivity to Triton X-100 and penicillin, attributable in part to increased murein hydrolase activity. Analysis of the chromosomal sequence flanking the transposon insertion site revealed that the gene disrupted in the mutant encodes an open reading frame of 147 amino acids. We named this gene rat , which stands for regulator of autolytic activity. Sequence analysis indicated that Rat is homologous to the MarR and, to a lesser extent, the SarA protein families. Mutations in rat resulted in decreased expression of known autolytic regulators lytSR , lrgAB and arlRS . Gel shift studies indicated that Rat binds to the lytRS and arlRS promoters, thus confirming Rat as a DNA-binding protein to these known repressors of autolytic activity. As anticipated, rat appears to be a negative regulator of autolysin genes including lytM and lytN . These data suggest that the rat gene product is an important regulator of autolytic activity in S. aureus .