Gemcitabine-loaded magnetic albumin nanospheres for cancer chemohyperthermia
Gemcitabine-loaded magnetic albumin nanospheres for cancer chemohyperthermia
复制标题
负载吉西他滨的磁性白蛋白纳米球用于癌症化疗热疗
DOI：
10.1007/s11051-013-1513-9
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发表时间：
2013-03-01
影响因子：
2.5
通讯作者：
Zhang, Dongsheng
中科院分区：
文献类型：
--
作者：
Li, Hongbo;Ke, Fei;Zhang, Dongsheng
在不伤害正常身体组织的情况下消除癌症仍然是医学界长期面临的挑战。为了实现这一目标，我们制备了包裹磁性纳米颗粒（Fe3O4）和抗肿瘤药物（吉西他滨，GEM）的纳米磁性白蛋白纳米球。磁性白蛋白纳米球（平均粒径约为224 nm）在交变磁场中具有良好的磁响应性，即使Fe3O4包裹在纳米球中。热力学测试表明，Fe3O4可以作为AMF下的加热源，使纳米球达到其稳定温度（45 ° C）。体外释药结果表明，纳米微球对吉西他滨具有明显的缓释作用。细胞毒性试验结果表明，该材料的毒性为1级，属于无细胞毒性。采用MTT法和流式细胞术检测GEM/Fe3O4白蛋白纳米球联合磁流体热疗对非小细胞肺癌细胞株GlC-82的抗肿瘤作用。与包埋GEM的纳米球组、包埋Fe3O4的纳米球结合MFH组以及不含MFH组的GEM/Fe3O4白蛋白纳米球相比，GEM/Fe3O4白蛋白纳米球具有更强的抗肿瘤作用。因此，GEM/Fe3O4白蛋白纳米球在肿瘤治疗中具有广阔的应用前景。
Eliminating cancer without harming normal body tissue remains a longstanding challenge in medicine. Toward this goal, we prepared nanosized magnetic albumin nanospheres encapsulating magnetic nanoparticles (Fe3O4) and antitumor drugs (Gemcitabine, GEM). Magnetic albumin nanospheres (average size ≈ 224 nm) had good magnetic responsiveness upon exposure to an alternating magnetic field even though Fe3O4was encased in nanospheres. Thermodynamic test showed that Fe3O4could serve as a heating source under AMF and lead the nanospheres to reach their steady temperature (45 °C). The release results in vitro indicated that nanospheres had an obvious effect of sustained release of GEM. The result of cytotoxicity assay showed that the toxicity of this material was classified as grade 1, which belongs to no cytotoxicity. The antitumor efficacy of the GEM/Fe3O4albumin nanospheres combined with magnetic fluid hyperthermia on non-small lung cancer cell line GlC-82 was examined by MTT assay and flow cytometry assay. Compared with nanospheres entrapping GEM group, nanospheres entrapping Fe3O4combined with MFH group, and GEM/Fe3O4albumin nanospheres without MFH group, the GEM/Fe3O4albumin nanospheres exhibited enhanced antitumor efficacy. Thus, the GEM/Fe3O4albumin nanospheres have promising applications in cancer treatment.