Action of FGMgCO3Ap-collagen composite in promoting bone formation
Action of FGMgCO3Ap-collagen composite in promoting bone formation
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DOI：
10.1016/s0142-9612(03)00414-9
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发表时间：
2003-12-01
期刊：
影响因子：
14
通讯作者：
Uchida, T
中科院分区：
文献类型：
--
作者：
Yamasaki, Y;Yoshida, Y;Uchida, T
为了改善骨替代材料的生物学性能，合成了含镁的功能梯度CO 3磷灰石晶体FGMgCO（3）Ap，并将其与去端胶原混合制成复合颗粒。放射性标记细胞粘附实验表明，小鼠成骨样细胞MC 3 T3 E1与FGMgCO（3）Ap-胶原复合材料的粘附程度优于与CO（3）Ap-胶原复合材料的粘附程度，远优于与钛板的粘附程度。将复合材料植入大鼠颅骨骨膜下，FGMgCO（3）Ap-胶原复合材料的代谢速度比CO（3）Ap-胶原复合材料快，复合材料与颅骨骨膜界面处新骨形成和成骨细胞排列更好。当将复合材料植入兔股骨时，观察到FGMgCO（3）Ap-胶原复合材料具有较高程度的骨密度和清晰的骨形成。这些结果表明，镁离子进入磷灰石晶体可能有助于加速成骨细胞粘附到磷灰石和促进骨形成，在分子水平上与成骨细胞交叉交谈。(C)2003 Elsevier Ltd.保留所有权利。
To improve the biological properties of materials as bone substitutes, functionally graded CO3 apatite crystals containing magnesium, FGMgCO(3)Ap, were synthesized to be mixed with atelocollagen and made into a composite pellet. A radio-labeled cell adhesion experiment showed that the degree of adherence of mouse MC3T3E1 osteoblast-like cells to the FGMgCO(3)Ap-collagen composite was better than to CO(3)Ap-collagen and much better than to the Ti plate. When the composites were implanted beneath the periosteum cranii of rats, the FGMgCO(3)Ap-collagen composite was metabolized faster than the CO(3)Ap-collagen composite and better formation of new bone and osteoblast arrangement at the interface between the composite and the periosteum cranii was observed. When the composites were implanted into the femur of rabbits, clear bone formation with a higher degree of bone density was observed for the FGMgCO(3)Ap-collagen composite. These results suggest that the Mg2+ ions taken into the apatite crystals may contribute to the acceleration of osteoblast adhesion to apatites and promote bone formation, cross-talking with osteoblasts at the molecular level. (C) 2003 Elsevier Ltd. All rights reserved.