Complete nucleotide sequence and organization of the mitogenome of the silk moth Caligula boisduvalii (Lepidoptera: Saturniidae) and comparison with other lepidopteran insects
Complete nucleotide sequence and organization of the mitogenome of the silk moth Caligula boisduvalii (Lepidoptera: Saturniidae) and comparison with other lepidopteran insects
复制标题
DOI：
10.1016/j.gene.2008.01.019
复制
发表时间：
2008-04-30
期刊：
影响因子：
3.5
通讯作者：
Kim, Iksoo
中科院分区：
文献类型：
--
作者：
Hong, Mee Yeon;Lee, Eun Mee;Kim, Iksoo
Caligula boisduvalii全长15360 bp的完整有丝分裂基因组具有与其他鳞翅目完整测序的有丝分裂基因组相同的基因排列和内容，但与大多数昆虫目的常见排列不同，这是由于tRNA（Met）移动到tRNA上游5'-位置（Ile）的结果。330 bp的富含A+ t的区域显然能够形成茎环结构，该结构在两端包含保守的侧翼序列。与其他鳞翅目昆虫的序列不同，C. boisduvalii COI的起始密码子似乎是TTG，这是一个罕见的，但显然可能的起始密码子。ATP8、ATP6、ND4L和ND6基因在其3'端与另一个PCG相邻，都含有形成发夹结构的潜在序列。这提示了这种结构对于成熟PCGs的mRNA的精确切割的重要性。对已有测序物种的系统发育分析支持了目前基于形态学的假设，即bombyco总科、pyralo总科和tortriciide总科是单系的（Obtectomera）。如前所述，家蚕科（家蚕和中国家蚕）和家蚕科（柞蚕和boisduvalii）形成了一个互惠的单系群。(c) 2008 Elsevier B.V.版权所有
The 15,360-bp long complete mitogenome of Caligula boisduvalii possesses a gene arrangement and content identical to other completely sequenced lepidopteran mitogenomes, but different from the common arrangement found in most insect order, as the result of the movement of tRNA(Met) to a position 5'-upstream of tRNA(Ile). The 330-bp A+T-rich region is apparently capable of forming a stem-and-loop structure, which harbors the conserved flanking sequences at both ends. Dissimilar to what has been seen in other sequenced lepidopteran insects, the initiation codon for C. boisduvalii COI appears to be TTG, which is a rare, but apparently possible initiation codon. The ATP8, ATP6, ND4L, and ND6 genes, which neighbor another PCG at their 3' end, all harbored potential sequences for the formation of a hairpin structure. This is suggestive of the importance of such structures for the precise cleavage of the mRNA of mature PCGs. Phylogenetic analyses of available sequenced species of Bombycoidea, Pyraloidea, and Tortricidea supported the morphology-based current hypothesis that Bombycoidea and Pyraloidea are monophyletic (Obtectomera). As previously suggested, Bombycidae (Bombyx mori and B. mandarina) and Satumiidae (Antheraea pernyi and C. boisduvalii) formed a reciprocal monophyletic group. (c) 2008 Elsevier B.V. All rights reserved.