Peptide-based carbon nanotubes for mitochondrial targeting
Peptide-based carbon nanotubes for mitochondrial targeting
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DOI：
10.1039/c3nr02694a
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发表时间：
2013-01-01
期刊：
影响因子：
6.7
通讯作者：
Bianco, Alberto
中科院分区：
文献类型：
--
作者：
Battigelli, Alessia;Russier, Julie;Bianco, Alberto
在本研究中，我们报告了基于肽的多壁碳纳米管（MWCNTs）靶向线粒体的设计和合成。靶向这些细胞内细胞器可能开辟了开发替代系统的途径，以通过提供治疗性寡核苷酸来解决与线粒体（mt）-DNA中基因突变相关的疾病。这种类型的核酸的线粒体递送的第一步是通过用众所周知的内源性线粒体靶向序列（MTS）共价官能化将MWCNT靶向线粒体。然后使用不同的显微镜技术，如宽场落射荧光显微镜，共聚焦激光扫描显微镜（CLSM）和透射电子显微镜（TEM）的亚细胞定位的缀合物，这是荧光标记，在小鼠RAW 264.7巨噬细胞和人HeLa细胞进行了研究。通过使用TEM分析分离的细胞器进一步证实MTS-MWCNT缀合物在线粒体中的定位。
In the present study, we report the design and synthesis of peptide-based-multi-walled carbon nanotubes (MWCNTs) to target mitochondria. Targeting these intracellular organelles might open the way to develop alternative systems to address diseases related to genetic mutations in mitochondrial (mt)-DNA, by delivering therapeutic oligonucleotides. The first step towards mitochondrial delivery of this type of nucleic acid was to target MWCNTs to mitochondria by covalent functionalization with a well-known endogenous mitochondrial targeting sequence (MTS). The subcellular localization of the conjugates, which were fluorescently labeled, in murine RAW 264.7 macrophages and human HeLa cells was then studied using different microscopy techniques, such as wide-field epifluorescence microscopy, confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM). The localization of the MTS-MWCNT conjugates into mitochondria was further confirmed by analyzing the isolated organelles using TEM.