Specific progesterone binding to a membrane protein and related nongenomic effects on Ca2+-fluxes in sperm.
Specific progesterone binding to a membrane protein and related nongenomic effects on Ca2+-fluxes in sperm.
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DOI：
10.1210/endo.140.12.7304
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发表时间：
1999-12
期刊：
影响因子：
4.8
通讯作者：
E. Falkenstein;M. Heck;D. Gerdes;D. Grube;M. Christ;Michael Weigel;M. Buddhikot;S. Meizel;M. Wehling
中科院分区：
文献类型：
--
作者：
E. Falkenstein;M. Heck;D. Gerdes;D. Grube;M. Christ;Michael Weigel;M. Buddhikot;S. Meizel;M. Wehling
类固醇的快速非基因组效应被认为是通过与经典的细胞内类固醇受体无关的膜受体传递的。在这种背景下，最近发现了一种可能的孕激素膜结合蛋白(MPR)。在这里，我们发现MPR-cDNAs在CHO细胞中的表达导致微粒体孕酮结合增加。这一结果反映在针对重组大肠杆菌MPR的抗体的效果上，该抗体抑制了黄体酮引发的精子中钙离子的快速增加。我们的结果支持这样的假设，即MPR代表第一个类固醇膜受体或它的一部分，参与快速、非基因组的类固醇信号传递。
Rapid, nongenomic effects of steroids are supposed to be transmitted by membrane receptors unrelated to the classic intracellular steroid receptors. In this context, a putative progesterone membrane binding protein (mPR) has been identified, recently. Here we show that expression of mPR-cDNA in CHO cells leads to increased microsomal progesterone binding. This result is mirrored by effects of an antibody raised against the recombinant E. coli mPR which suppressed the rapid progesterone-initiated Ca2+ increase in sperm. Our results support the assumption that mPR represents the first steroid membrane receptor or a part of it involved in rapid, nongenomic steroid signalling.