Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus)
Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus)
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DOI：
10.1016/j.aquaculture.2005.01.031
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发表时间：
2005-09-12
期刊：
影响因子：
4.5
通讯作者：
Hong, YH
中科院分区：
文献类型：
--
作者：
Chen, SL;Ren, GC;Hong, YH
连续细胞系TEC（大菱鲆胚胎细胞系）是从养殖海鱼大菱鲆（Scophamus maximus）原肠胚阶段的胚胎建立的，已培养200多天，传代60多次。 TEC 细胞在补充有抗生素、胎牛血清 (FBS)、鲈鱼血清 (SPS) 和碱性成纤维细胞生长因子 (bFGF) 的 DMEM 培养基中培养。细胞小而圆，培养中生长活跃、稳定。研究了温度、胎牛血清 (FBS) 浓度和 bFGF 对 TEC 细胞生长的影响。细胞在24-30℃的温度范围内生长良好，但在低于18℃的温度下生长速率降低。TEC细胞在含有15％FBS的培养基中的生长速率高于含有7.5％FBS的培养基中的生长速率。培养基中添加 bFGF 显着提高了 TEC 细胞的生长速度。染色体分析显示TEC细胞具有正常的二倍体核型，2n=4m+12st+28t。细胞培养物冷冻保存后可获得高存活率。 (c) 2005 Elsevier B.V. 保留所有权利。
A continuous cell line, TEC (turbot embryonic cell line), was established from embryos at the gastrula stage of a cultured marine fish, turbot (Scophthalmus maximus), and has been cultured for more than 200 days with more than 60 passages. The TEC cells were cultured in DMEM medium supplemented with antibiotics, fetal bovine serum (FBS), sea perch serum (SPS), and basic fibroblast growth factor (bFGF). The cells were small and round, and grew actively and stably in culture. Effect of temperature, fetal bovine serum (FBS) concentration, and bFGF on the growth of TEC cells was examined. The cells grew well in the temperature range of 24-30 degrees C, but have a reduced growth rate at temperatures below 18 degrees C. The growth rate of TEC cells in medium containing 15% FBS was higher than that in medium containing 7.5% FBS. Addition of bFGF to the medium significantly increased the growth rate of TEC cells. Chromosome analysis revealed that TEC cells have a normal diploid karyotype with 2n=4m+12st+28t. High survival rate has been obtained after cryopreservation of cell cultures. (c) 2005 Elsevier B.V. All rights reserved.