Estrogen increases spine density in ventromedial hypothalamic neurons of peripubertal rats.
Estrogen increases spine density in ventromedial hypothalamic neurons of peripubertal rats.
复制标题
雌激素增加青春期大鼠腹内侧下丘脑神经元的脊柱密度。
DOI：
10.1159/000125915
复制
发表时间：
1991
影响因子：
4.1
通讯作者：
McEwen,BS
中科院分区：
文献类型：
--
作者：
Segarra,AC;McEwen,BS
采用单切片高尔基体浸渍技术，研究了去性腺幼年大鼠和青春期大鼠下丘脑腹内侧核（VMN）神经元的性别差异。雌激素处理对VMN神经元的影响也进行了研究。幼年大鼠在16日龄时切除性腺，青春期大鼠在36日龄时切除性腺。手术后5天，向大鼠注射苯甲酸雌二醇（20 µg/kg）或芝麻油2天，然后进行灌注，并对大脑进行高尔基体浸渍处理。苯甲酸雌二醇处理显著增加幼年和青春期前后雄性和雌性大鼠的树突和索马棘密度。在油和雌二醇处理的大鼠中观察到性别差异，雌性大鼠表现出更高的树突棘密度和对雌激素引发的更大反应。此外，树突状细胞和索马棘密度显着较高的青少年与青春期大鼠。在树突和索马棘密度以及对苯甲酸雌二醇治疗的反应中观察到的性别差异可能是由于性类固醇的组织效应。
Using the single-section Golgi impregnation technique, sex differences in hypothalamic ventromedial (VMN) neurons of gonadectomized juvenile and peripubertal rats were assessed. The effect of estrogen treatment on VMN neurons was also investigated. Juvenile rats were gonadectomized at 16 days of age and peripubertal rats at 36 days. At 5 days after surgery, the rats were injected with estradiol benzoate (20 µg/kg) or sesame oil for 2 days after which they were perfused and the brains processed for Golgi impregnation. Estradiol benzoate treatment significantly increased dendritic and soma spine density in juvenile and peripubertal male and female rats. A sex difference was observed in oil- and in estradiol-treated rats, with females exhibiting higher dendritic spine density and a greater response to estrogen priming. Moreover, dendritic and soma spine density was significantly higher in juvenile versus peripubertal rats. It is possible that the sex difference observed in dendritic and soma spine density and in the response to estradiol benzoate treatment is due to an organizational effect of sex steroids.