Genomic characterization of three novel Desulfobacterota classes expand the metabolic and phylogenetic diversity of the phylum
Genomic characterization of three novel Desulfobacterota classes expand the metabolic and phylogenetic diversity of the phylum
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DOI：
10.1111/1462-2920.15614
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发表时间：
2021-06-05
影响因子：
5.1
通讯作者：
Youssef, Noha H.
中科院分区：
文献类型：
--
作者：
Murphy, Chelsea L.;Biggerstaff, James;Youssef, Noha H.
我们报告了三个新的纲在门脱硫菌的基因组特征。其中一类（拟命名为Candidatus ' anaerofrophillalia '）的特点是异养生长能力，要么通过发酵，要么利用聚硫、四硫酸盐或硫代硫酸盐作为电子受体。在缺乏有机碳源的情况下，“厌氧嗜氧菌”的成员也可以通过Wood-Ljungdahl （WL）途径自养生长，并使用氢或Fe（II）作为电子供体。第二类（拟命名为Candidatus 'Anaeropigmentia'）的特点是其在低氧浓度下生长的能力，以及合成甲基/烷基载体CoM的能力，这种能力在古细菌中普遍存在，但在细菌领域却很少见。色素沉着是从类胡萝卜素（番茄红素）生产能力推断出来的。第三类（拟命名为Candidatus 'Zymogenia'）具有发酵异养生长能力、底物范围广以及部分成员适应高盐生境的特点。分布格局分析表明，这3个类在多种生境中都是罕见的群落成员，它们更倾向于厌氧陆地、淡水和海洋环境，而不是含氧环境（如中上层海洋和农业用地）。观察到一些候选菌（Candidatus 'Zymogenia）类成员对高盐环境（如高盐微生物垫和泻湖）的特殊偏好。
We report on the genomic characterization of three novel classes in the phylum Desulfobacterota. One class (proposed name Candidatus 'Anaeroferrophillalia') was characterized by heterotrophic growth capacity, either fermentatively or utilizing polysulfide, tetrathionate or thiosulfate as electron acceptors. In the absence of organic carbon sources, autotrophic growth via the Wood-Ljungdahl (WL) pathway and using hydrogen or Fe(II) as an electron donor is also inferred for members of the 'Anaeroferrophillalia'. The second class (proposed name Candidatus 'Anaeropigmentia') was characterized by its capacity for growth at low oxygen concentration, and the capacity to synthesize the methyl/alkyl carrier CoM, an ability that is prevalent in the archaeal but rare in the bacterial domain. Pigmentation is inferred from the capacity for carotenoid (lycopene) production. The third class (proposed name Candidatus 'Zymogenia') was characterized by fermentative heterotrophic growth capacity, broad substrate range and the adaptation of some of its members to hypersaline habitats. Analysis of the distribution pattern of all three classes showed their occurrence as rare community members in multiple habitats, with preferences for anaerobic terrestrial, freshwater and marine environments over oxygenated (e.g. pelagic ocean and agricultural land) settings. Special preference for some members of the class Candidatus 'Zymogenia' for hypersaline environments such as hypersaline microbial mats and lagoons was observed.