Phosphoinositide 3-kinase is required for human adipocyte differentiation in culture
Phosphoinositide 3-kinase is required for human adipocyte differentiation in culture
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DOI：
10.1038/sj.ijo.0802961
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发表时间：
2005-08-01
影响因子：
4.9
通讯作者：
Sorisky, A
中科院分区：
文献类型：
--
作者：
Aubin, D;Gagnon, A;Sorisky, A
目的：磷脂酰肌醇3-激酶(PI3K)是小鼠脂肪细胞分化所必需的。然而，最近的一份报告得出结论，PI3K不参与人前脂肪细胞向脂肪细胞的分化。我们重新研究了PI3K在人前脂肪细胞分化中的作用，招募了更多的患者，并使用了更多的成脂指标。方法：从9名患者分离的人前脂肪细胞在100 nm Wortmannin存在或不存在的情况下被诱导分化。结果：PI3K抑制人前脂肪细胞向脂肪细胞分化的过程中，三酰甘油积累和脂肪合成酶蛋白表达均受到明显抑制，但对脂联素蛋白表达无明显影响。
OBJECTIVE: Phosphoinositide 3-kinase (PI3K) is required for murine adipocyte differentiation. However, a recent report concluded that PI3K was not involved in the differentiation of human preadipocytes into adipocytes. We have re-examined the role of PI3K in human preadipocyte differentiation, enrolling more patients and using more adipogenic indices.METHODS: Human preadipocytes, isolated from nine patients, were induced to differentiate in the presence or absence of 100 nM wortmannin. After 12-15 days, triacylglycerol accumulation and the expression of adipogenic markers ( fatty acid synthase and adiponectin) were measured.RESULTS: A significant inhibition in triacylglycerol accumulation and in the induction of fatty acid synthase protein expression was observed, but there was no effect on adiponectin protein expression.CONCLUSION: Inhibition of PI3K reduces the differentiation of human preadipocytes into adipocytes, suggesting a role for this enzyme in the human adipogenic process.