Effects of nitric oxide synthase inhibition on cutaneous vasodilation in response to acupuncture stimulation in humans
Effects of nitric oxide synthase inhibition on cutaneous vasodilation in response to acupuncture stimulation in humans
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DOI：
10.1136/acupmed-2012-010177
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发表时间：
2013-03-01
影响因子：
2.5
通讯作者：
Wakayama, Ikuro
中科院分区：
文献类型：
--
作者：
Kimura, Kenichi;Takeuchi, Hayato;Wakayama, Ikuro
目的通过观察一氧化氮（nitric oxide，NO）和轴突反射性血管舒张反应在针刺刺激皮肤血管舒张中的作用，探讨针刺刺激皮肤血管舒张的机制。通过皮内微透析（方案1; n=7），通过施用N-G-硝基-L-精氨酸甲酯盐酸盐（L-NAME，20 mM）、NO合酶抑制剂或林格氏溶液（对照部位）来研究NO的作用。通过比较用“局部麻醉剂的低共熔混合物”（EWA）乳膏（2.5%利多卡因和2.5%丙胺卡因）处理的部位与未处理部位（对照部位）的血管舒张来研究局部感觉神经元的轴突反射血管舒张作用（方案2; n=10）。基线记录5 min后，针刺PC 4和邻近PC 4的对照部位10 min，针刺刺激后扫描60 min。采用激光多普勒血流灌注成像评价皮肤血流量（SkBF）。根据SkBF与平均动脉血压的比值计算皮肤血管传导性（CVC）。与对照部位相比，针刺刺激后给予L-NAME的部位显示CVC反应显著降低（给予林格氏溶液）（p <0.05）结论针刺刺激引起的皮肤血管舒张反应可能不是通过轴突反射发生的。相反，NO机制似乎有助于血管舒张反应。
Objectives The aim of the present study was to elucidate the mechanism of cutaneous vasodilation following acupuncture stimulation by investigating the roles of nitric oxide (NO) and axon reflex vasodilation.Methods The subjects were 17 healthy male volunteers. The role of NO was investigated by administering N-G-nitro-L-arginine methyl ester hydrochloride (L-NAME, 20 mM), an NO synthase inhibitor or Ringer's solution (control site), via intradermal microdialysis (protocol 1; n=7). The role of axon reflex vasodilation by local sensory neurones was investigated by comparing vasodilation at sites treated with 'eutectic mixture of local anaesthetics' (EWA) cream (2.5% lidocaine and 2.5% prilocaine) with untreated sites (control site) (protocol 2; n=10). After 5 min of baseline recording, acupuncture was applied to PC4 and a control site in proximity to PC4 for 10 min and scanning was performed for 60 min after acupuncture stimulation. Skin blood flow (SkBF) was evaluated by laser Doppler perfusion imaging. Cutaneous vascular conductance (CVC) was calculated from the ratio of SkBF to mean arterial blood pressure.Results In the first protocol, sites administered L-NAME showed significant reductions in CVC responses following acupuncture stimulation compared to control sites (administered Ringer's solution) (p0.05).Conclusions These data suggest that cutaneous vasodilation in response to acupuncture stimulation may not occur through an axon reflex as previously reported. Rather, NO mechanisms appear to contribute to the vasodilator response.