Restriction fragment length polymorphism of polymerase chain reaction products applied to the differentiation of poultry campylobacters for epidemiological investigations
Restriction fragment length polymorphism of polymerase chain reaction products applied to the differentiation of poultry campylobacters for epidemiological investigations
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DOI：
10.1016/s0034-5288(96)90013-2
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发表时间：
1996-03-01
影响因子：
2.4
通讯作者：
Newell, DG
中科院分区：
文献类型：
--
作者：
Ayling, RD;Woodward, MJ;Newell, DG
应用限制性片段长度多态性（Rnp）技术对空肠弯曲菌的fluA和flaB基因进行了分型。该技术通过使用代表28个血清型的空肠弯曲菌菌株进行了验证，并且它也可以应用于大肠杆菌。从这些菌株中观察到12个不同的RFLP图谱，但RFLP图谱与血清型之间没有直接关系。从15个地理位置不同的肉鸡群的弯曲杆菌分离株135进行了调查。用RFLP方法可对所有菌株进行分型。尽管数据表明涉及几种血清型，但来自大多数鸡群的分离株具有单一RFLP图谱。虽然进一步的限制性内切酶分析可能已经证明了这些菌株的特征变异，但更有可能的是抗原变异可能发生在基因型相关的弯曲杆菌中。因此，血清分型可能为兽医流行病学目的提供相互矛盾的信息。该RFLP分型方案似乎为调查鸡弯曲杆菌的来源和传播途径提供了合适的工具。
A technique for subtyping Camplobacter jejuni isolates has been developed by using the restriction fragment length polymorphism (Rnp) of polymerase chain reaction (PCR) products of the fluA and flaB genes. The technique was validated by using strains representing 28 serotypes of C jejuni and it may also be applied to C coli. From these strains 12 distinct RFLP profiles were observed but there was no direct relationship between the RFLP profile and the serotype. One hundred and thirty-five campylobacter isolates from 15 geographically distinct broiler flocks were investigated. All the isolates could be subtyped by using the RFLP method. Isolates from most of the flocks had a single RFLP profile despite data indicating that several serotypes were involved. Although it is possible that further restriction analysis may have demonstrated profile variations in these strains, it is more likely that antigenic variation can occur within genotypically related campylobacters. As a result, serotyping may give conflicting information for veterinary epidemiological purposes. This RFLP typing scheme appears to provide a suitable tool for the investigation of the sources and routes of transmission of campylobacters in chickens.