The Evolutionary History of Sarco(endo)plasmic Calcium ATPase (SERCA)
The Evolutionary History of Sarco(endo)plasmic Calcium ATPase (SERCA)
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DOI：
10.1371/journal.pone.0052617
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发表时间：
2012-12-20
期刊：
影响因子：
3.7
通讯作者：
Cristescu, Melania E.
中科院分区：
文献类型：
--
作者：
Altshuler, Ianina;Vaillant, James J.;Cristescu, Melania E.
在广泛的分类群中研究生理必需基因家族的系统发育关系可以揭示其家族扩张背后的关键基因复制事件，因此对功能基因组学研究具有重要意义。p型II型ATP酶代表了一个ATP驱动的转运蛋白家族，这些转运蛋白通过细胞膜移动离子，包括Na+/K+转运蛋白、H+/K+转运蛋白和质膜Ca2+泵。在这里，我们研究了一个这样的转运体，Sarco（内do）质网钙atp酶（SERCA）的进化史，它通过主动泵送Ca2+进入Sarco（内do）质网来维持细胞内钙的稳态。我们基于蛋白质的真核生物系统发育分析揭示了SERCA蛋白的两个单系分支，一个包含动物、真菌和植物，另一个包含植物和原生生物。我们的分析表明，脊椎动物中已知的三种SERCA蛋白是在与被囊动物分化之后，但在鱼类和四足动物分离之前，通过两次主要的基因复制事件产生的。在植物中，我们恢复了两个SERCA分支，一个是后生动物的姐妹群，另一个是顶复合体的分支，表明在早期真核生物祖先中存在古老的复制，随后在Opisthokonta中丢失了一个副本，在原生生物中丢失了另一个副本，然后在植物中保留了这两个副本。我们还报道了包括被囊动物和水蛭在内的无脊椎动物分类群中相对较新的和独立的基因复制事件。因此，似乎古代和最近的基因复制事件在整个真核生物领域普遍存在的基因家族的进化中发挥了重要作用。
Investigating the phylogenetic relationships within physiologically essential gene families across a broad range of taxa can reveal the key gene duplication events underlying their family expansion and is thus important to functional genomics studies. P-Type II ATPases represent a large family of ATP powered transporters that move ions across cellular membranes and includes Na+/K+ transporters, H+/K+ transporters, and plasma membrane Ca2+ pumps. Here, we examine the evolutionary history of one such transporter, the Sarco(endo)plasmic reticulum calcium ATPase (SERCA), which maintains calcium homeostasis in the cell by actively pumping Ca2+ into the sarco(endo)plasmic reticulum. Our protein-based phylogenetic analyses across Eukaryotes revealed two monophyletic clades of SERCA proteins, one containing animals, fungi, and plants, and the other consisting of plants and protists. Our analyses suggest that the three known SERCA proteins in vertebrates arose through two major gene duplication events after the divergence from tunicates, but before the separation of fishes and tetrapods. In plants, we recovered two SERCA clades, one being the sister group to Metazoa and the other to Apicomplexa clade, suggesting an ancient duplication in an early eukaryotic ancestor, followed by subsequent loss of one copy in Opisthokonta, the other in protists, and retention of both in plants. We also report relatively recent and independent gene duplication events within invertebrate taxa including tunicates and the leech Helobdella robusta. Thus, it appears that both ancient and recent gene duplication events have played an important role in the evolution of this ubiquitous gene family across the eukaryotic domain.