Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats
Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats
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DOI：
10.1039/c9fo00460b
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发表时间：
2019-07-01
期刊：
影响因子：
6.1
通讯作者：
Xue, Xiaofeng
中科院分区：
文献类型：
--
作者：
Wang, Kai;Wan, Zhengrui;Xue, Xiaofeng
由药用植物生产的蜂蜜对人类健康有着巨大的前景。在此，我们测定了一种来自夏枯草 (PVH) 的新型单花蜂蜜的化学成分和胃肠道保护作用。 PVH样品的理化参数（水分、糖分、pH、蛋白质含量、淀粉酶活性和羟甲基糠醛）符合欧盟法规和中国国家标准规定的标准。通过使用二极管阵列检测器和飞行时间串联质谱检测 (HPLC-DAD/Q-TOF-MS) 的高效液相色谱法对 15 种酚类化合物进行了鉴定和定量。迷迭香酸被发现是 PVH 鉴定的潜在标志物。使用葡聚糖硫酸钠 (DSS) 诱导的急性结肠炎模型，我们证明给予 PVH（5 g/kg b.w.，口服）可显着降低大鼠的疾病活动指数并减轻结肠组织病理学变化。 PVH 还调节结肠炎大鼠的肠道微生物群组成，逆转拟杆菌/厚壁菌门比例的增加并恢复乳杆菌属。 DSS 攻击的大鼠群体。本研究结果提供了PVH的基础数据，支持其未来在预防结肠炎中的应用。
Honeys produced from medicinal plants hold great promise for human health. Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH). The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards. Fifteen phenolic compounds were identified and quantified via high-performance liquid chromatography with a diode array detector and with time of flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS). Rosmarinic acid was found to be a potential marker for PVH identification. Using a dextran sulfate sodium (DSS)-induced acute colitis model, we demonstrated that the administration of PVH (5 g per kg b.w., p.o.) significantly decreased the disease activity index and mitigated colonic histopathological changes in rats. PVH also modulated the gut microbiota composition in the colitic rats, reversing the increase in the Bacteroidetes/Firmicutes ratio and restoring Lactobacillus spp. populations in DSS-challenged rats. The results of this study provide fundamental data on PVH, supporting its future application in the prevention of colitis.