Analysis of Trace and Major Elements in Bronchoalveolar Lavage Fluid of Mycoplasma Bronchopneumonia in Calves
Analysis of Trace and Major Elements in Bronchoalveolar Lavage Fluid of Mycoplasma Bronchopneumonia in Calves
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DOI：
10.1007/s12011-011-9180-0
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发表时间：
2012-02
影响因子：
3.9
通讯作者：
Kazuyuki Suzuki;H. Higuchi;H. Iwano;J. Lakritz;K. Sera;M. Koiwa;K. Taguchi
中科院分区：
文献类型：
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作者：
Kazuyuki Suzuki;H. Higuchi;H. Iwano;J. Lakritz;K. Sera;M. Koiwa;K. Taguchi
本研究的目的是评价直接测定支气管肺泡灌洗液中微量和常量元素浓度的可靠性和有效性，这些样品来自支气管肺炎支原体感染的荷斯坦犊牛（n= 21）和健康对照组（n= 20）。使用标准检查方法在支气管镜检查期间获得样本。通过粒子诱导X射线发射，共检测到18种元素（铝、溴、钙、氯、铬、铜、铁、钾、镁、锰、钼、镍、磷、硫、硅、锶、钛和锌）。平均溴、铁、钾、镁和磷浓度在患有支气管肺炎的小牛中高于对照组（p< 0.05）。结果表明，与健康犊牛相比，高钙、高锌、高锌铜比（P< 0.001）。根据受试者工作特征曲线，我们提出了诊断支原体肺炎的锌铜比值的诊断临界点为8.676。我们的研究结果表明，支气管肺泡灌洗液的元素组成的评估是一个有前途的诊断工具支原体支气管肺炎。
The aim of this study was to evaluate the reliability and effectiveness of direct determination of trace and major element concentrations in bronchoalveolar lavage fluid samples from Holstein calves withMycoplasmabronchopneumonia (n= 21) and healthy controls (n= 20). The samples were obtained during bronchoscopy using a standard examination method. A total of 18 elements (aluminum, bromine, calcium, chlorine, chromium, copper, iron, potassium, magnesium, manganese, molybdenum, nickel, phosphorous, sulfur, silicon, strontium, titanium, and zinc) were detected by particle-induced X-ray emission. The average bromine, iron, potassium, magnesium, and phosphorous concentrations were higher in calves with bronchopneumonia than in controls (p< 0.05). They were found to have higher amounts of calcium and zinc, and a higher zinc–copper ratio than that in healthy calves (p< 0.001). Based on the receiver operating characteristics curves, we propose a diagnostic cutoff point for zinc–copper ratio for identification ofMycoplasmapneumonia of 8.676. Our results indicate that assessment of the elemental composition of broncholaveolar lavage fluid is a promising diagnostic tool forMycoplasmabronchopneumonia.