Remote Ischemic Pre-Conditioning (RIPC).
Remote Ischemic Pre-Conditioning (RIPC).
复制标题
远程缺血预调节（RIPC）。
DOI：
10.1007/978-1-0716-2835-5_2
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Zhu,Hua
中科院分区：
文献类型：
--
作者：
Lee,KyungEun;Kim,Jongsoo;Park,KiHo;Ma,Jianjie;Zhu,Hua
缺血预处理已被证明可以保护心脏免受缺血/再灌注（I/R）诱导的心脏损伤。但在临床上并不可行。许多研究者试图在骨骼肌中引入短暂的I/R来模拟心脏缺血预处理，称为远程缺血预处理（RIPC）。我们小组和其他小组的研究表明，RIPC诱导骨骼肌释放细胞因子（肌因子）用于组织保护。肌因子在损伤后的修复、炎症和免疫反应中起核心作用。因此，RIPC的详细方案可能有助于研究人员研究RIPC介导的组织保护和串扰的机制。在这里，我们描述了详细的RIPC方案，并显示了RIPC进入血液后MG 53的分泌。
Ischemic pre-conditioning has been shown to protect hearts against ischemia/reperfusion (I/R)-induced cardiac injury. However, it is not feasible in clinic. Many researchers have tried to introduce brief I/R in skeletal muscle to mimic cardiac ischemic pre-conditioning, called remote ischemia pre-conditioning (RIPC). Studies from our group and other groups have shown that RIPC induces the release of cytokines from skeletal muscle (myokines) for tissue protection. Myokines play a central role in repair, inflammatory, and immune responses after injury. Thus, the detailed protocol for RIPC might be useful for researchers to study mechanisms underlying RIPC-mediated tissue protection and crosstalk. Here, we describe a detailed RIPC protocol and show MG53 secretion after RIPC into the blood.