Identification of three mouse μ-opioid receptor (MOR) gene (Oprm1) splice variants containing a newly identified alternatively spliced exon
Identification of three mouse μ-opioid receptor (MOR) gene (Oprm1) splice variants containing a newly identified alternatively spliced exon
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DOI：
10.1016/j.gene.2006.10.017
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发表时间：
2007-02-15
期刊：
影响因子：
3.5
通讯作者：
Berrettini, Wade H.
中科院分区：
文献类型：
--
作者：
Doyle, Glenn A.;Sheng, X. Rebecca;Berrettini, Wade H.
目前认为小鼠 It-阿片受体基因 Oprm 1 含有 17 个可变剪接外显子，这些外显子产生 24 个编码受体的至少 11 个吗啡结合亚型的剪接变体。在这里，我们鉴定了三种新的 MOR 剪接变体，其中包含先前未描述的外显子（外显子 18），并提供了它们在两种小鼠品系中表达的证据。含有新鉴定的外显子 18 的转录物编码两种新的推定 mu-阿片受体异构体：MOR-1V 和 MOR-1W。在小鼠 Oprm1 中，外显子 18 位于所描述的外显子 10 和 6 之间。外显子 18 似乎在大鼠基因组中的外显子 4 和 7 之间保守。对非冗余 GenBank 数据库的 BLAST 搜索表明，人 OPRM1 也可能包含外显子 18。通过 RT-PCR 对小鼠大脑 mRNA 的分析表明，MOR-1Vii 转录物在分析的大脑的所有区域中都有表达，而MOR-1Vi 转录本仅限于丘脑和纹状体。 MOR-1W 转录本在下丘脑、丘脑和纹状体中表达最高。总之，我们鉴定了三种大脑表达的、选择性剪接的小鼠 MOR 剪接变体，其中包含新的外显子并编码新的推定 MOR 亚型 MOR-1V 和 MOR-1W。 (c) 2006 Elsevier B.V. 保留所有权利。
The mouse It-opioid receptor gene, Oprm 1, is recognized currently to contain 17 alternatively spliced exons that generate 24 splice variants encoding at least I I morphine-binding isoforms of the receptor. Here, we identify three new MOR splice variants that contain a previously undescribed exon, exon 18, and provide evidence that they are expressed in two mouse strains. The transcripts containing the newly identified exon 18 encode two new putative mu-opioid receptor isofonns, MOR-1V and MOR-1W. In mouse Oprm1, exon 18 is located between the described exons 10 and 6. Exon 18 appears to be conserved in the rat genome between exons 4 and 7. A BLAST search of the non-redundant GenBank database suggests that human OPRM1 may also contain exon 18. Analysis of mouse brain mRNA by RT-PCR suggests that MOR-1Vii transcripts are expressed in all areas of the brain analyzed, whereas expression of MOR-1Vi transcripts was restricted to thalamus and striatum. MOR-1W transcripts are expressed most highly in the hypothalamus, thalamus and striatum. In summary, we have identified three brain expressed, alternatively spliced mouse MOR splice variants containing a novel exon and encoding new putative MOR isoforms, MOR-1V and MOR-1W. (c) 2006 Elsevier B.V. All rights reserved.