IMMUNOHISTOCHEMICAL ANALYSIS OF THE CELLULAR INFILTRATE IN MULTIPLE-SCLEROSIS LESIONS
IMMUNOHISTOCHEMICAL ANALYSIS OF THE CELLULAR INFILTRATE IN MULTIPLE-SCLEROSIS LESIONS
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DOI：
10.1002/ana.410190610
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发表时间：
1986-06-01
影响因子：
11.2
通讯作者：
WEINER, HL
中科院分区：
文献类型：
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作者：
HAUSER, SL;BHAN, AK;WEINER, HL
使用一组与 T 细胞和 T 细胞亚群、B 细胞和 Ia (HLA-DR) 抗原反应的单克隆抗体，对 16 例进行性多发性硬化症患者的死后大脑和两份急性脱髓鞘疾病患者的活检标本进行免疫组织化学染色。淋巴细胞血管周围袖口在活动斑块的边缘最为突出，偶尔可见于没有脱髓鞘或巨噬细胞浸润证据的区域。血管周围袖带主要由 T 细胞和 Ia+ 细胞组成，还有许多 T8+ 细胞和数量不等的 T4+ 细胞。袖口中的 T8/T4 比率在 1:1 至 50:1 之间变化。在正常出现的白质中，袖带稀疏并且主要是 T8+。 T 细胞在实质中的分布类似于血管周围袖带中所见的分布，即主要是 T8+ 细胞和数量不等的 T4+ 细胞。许多 Ia+ 细胞存在于活动性病变中，根据组织学标准，这些细胞中的大多数似乎是巨噬细胞。组织巨噬细胞也被抗 T4 抗体轻微染色。使用 T4 和 Leu3a 单克隆抗体测定，没有大脑中 T4+ 细胞数量多于 T8+ 细胞。 B1+细胞很少见。这些结果表明，多发性硬化症中的细胞浸润主要由 T 细胞和巨噬细胞组成，并且与 T4+ 细胞相比，T8+ 细胞的比例过高。
Immunohistochemical staining of 16 brains post mortem from patients with progressive multiple sclerosis and of two biopsy specimens from patients with acute demyelinating disease was performed using a panel of monoclonal antibodies reactive with T cells and T-cell subsets, B cells, and Ia (HLA-DR) antigens. Lymphocytic perivascular cuffs were most prominent at the edge of active plaques and were occasionally seen in areas with no evidence of demyelination or macrophage infiltration. Perivascular cuffs consisted predominantly of T cells and Ia+ cells, with many T8+ cells and variable numbers of T4+ cells. T8/T4 ratios in cuffs varied between 1:1 and 50:1. In normal-appearing white matter, cuffs were sparse and were predominantly T8+. The distribution of T cells in the parenchyma resembled that seen in perivascular cuffs, namely, predominantly T8+ cells and variable numbers of T4+ cells. Many Ia+ cells were present in active lesions, and the majority of these cells appeared, by histological criteria, to be macrophages. Tissue macrophages were also stained lightly by the anti-T4 antibody. No brain had more T4+ than T8+ cells, determined using both T4 and Leu3a monoclonal antibodies. B1+ cells were rare. These results suggest that the cellular infiltrate in multiple sclerosis consists predominantly of T cells and macrophages and that there is an overrepresentation of T8+ cells compared with T4+ cells.