Immunocytochemical localization of caveolin-3 in the synoviocytes of the rat temporomandibular joint during development.
Immunocytochemical localization of caveolin-3 in the synoviocytes of the rat temporomandibular joint during development.
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DOI：
10.1002/ar.20655
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发表时间：
2008-03
影响因子：
--
通讯作者：
M. Niwano;K. Nozawa‐Inoue;Akiko Suzuki;N. Ikeda;R. Takagi;T. Maeda
中科院分区：
文献类型：
--
作者：
M. Niwano;K. Nozawa‐Inoue;Akiko Suzuki;N. Ikeda;R. Takagi;T. Maeda
小窝蛋白--小窝蛋白-1、-2、-3(小窝1、2、3)是小窝的主要成分，具有不同的功能。我们最近对颞下颌关节(TMJ)的研究发现，Cav1和Cav3在一些滑膜成纤维样细胞样B细胞中表达，这些细胞具有发育良好的小窝。然而，Cav3在B细胞分化和成熟过程中的作用尚不清楚。因此，本研究用免疫细胞化学技术检测了出生后大鼠TMJ滑膜衬里细胞中Cav3定位的时序性变化。观察发现，未成熟的B细胞在出生后第5天有少量的Cav1小凹，但缺乏Cav3蛋白(P5)。在P14，Cav3免疫阳性的B型细胞首先出现在滑膜衬里层。随着闭塞变得活跃，它们的数量和免疫强度从P14到P21逐渐增加。免疫电子显微镜和热休克蛋白25(HSP25)和Cav3双重免疫标记显示，共表达的B细胞出现粗面内质网和大量小凹，而HSP25免疫反应阴性的B细胞几乎没有这些。结果提示，Cav3的表达反映了B型滑膜细胞的分化，其表达与外加功能刺激密切相关。
Caveolins -- caveolin-1, -2, -3 (Cav1, 2, 3) -- are major components of caveolae, which have diverse functions. Our recent study on the temporomandibular joint (TMJ) revealed expressions of Cav1 and muscle-specific Cav3 in some synovial fibroblast-like type B cells with well-developed caveolae. However, the involvement of Cav3 expression in the differentiation and maturation of type B cells remains unclear. The present study therefore examined the chronological alterations in the localization of Cav3 in the synovial lining cells of the rat TMJ during postnatal development by immunocytochemical techniques. Observations showed immature type B cells possessed a few caveolae with Cav1 but lacked Cav3 protein at postnatal day 5 (P5). At P14, Cav3-immunopositive type B cells first appeared in the synovial lining layer. They increased in number and immunointensity from P14 to P21 as occlusion became active. In immunoelectron microscopy and double immunolabeling with heat shock protein 25 (Hsp25) and Cav3, coexpressed type B cells developed rough endoplasmic reticulum and numerous caveolae, while the Cav3-immunonegative type B cell with Hsp25 immunoreaction possessed few of these. Results suggest that Cav3 expression, which is closely related to added functional stimuli, reflects the differentiation of the type B synoviocytes.