Global protein expression analysis in apicomplexan parasites: Current status
Global protein expression analysis in apicomplexan parasites: Current status
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DOI：
10.1002/pmic.200401161
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发表时间：
2005-03-01
期刊：
影响因子：
3.4
通讯作者：
Flowers, SA
中科院分区：
文献类型：
--
作者：
Belli, SL;Walker, RA;Flowers, SA
Apicomplexa门的成员是重要的原生动物寄生虫，它们会导致一些最严重的疾病，在某些情况下，会导致人类和动物的致命疾病。它们包括来自疟原虫属、弓形虫属、艾美耳球虫属、新孢子虫属、隐孢子虫属、巴贝斯虫属和泰勒虫属的物种。这些病原体在全球范围内的医疗、兽医和经济影响是巨大的。虽然化学和免疫预防策略可以用来控制其中一些寄生虫，但它们是不够的。目前，迫切需要设计新的疫苗或化疗药物来治疗尖端复合体疾病。利用凝胶或非凝胶蛋白质分离技术结合质谱学和生物信息学的高通量全球蛋白质表达分析为在这些病原体中识别新的药物和疫苗靶标提供了一种手段。基于蛋白质鉴定的顶端复合体寄生虫蛋白质组学项目目前正在进行中，在疟疾寄生虫恶性疟原虫方面取得了最重大的进展。最近，弓形虫、犬新孢子虫速殖子和柔嫩艾美耳球虫子孢子以及柔嫩艾美耳球虫微线体的双向凝胶电泳图已经初步绘制出来。本文将比较蛋白质组学在顶端复合体寄生虫全球蛋白质表达分析中的地位，并讨论与这些研究相关的挑战。
Members of the phylum Apicomplexa are important protozoan parasites that cause some of the most serious, and in some cases, deadly diseases in humans and animals. They include species from the genus Plasmodium, Toxoplasma, Eimeria, Neospora, Cryptosporidium, Babesia and Theileria. The medical, veterinary and economic impact of these pathogens on a global scale is enormous. Although chemo- and immuno-prophylactic strategies are available to control some of these parasites, they are inadequate. Currently, there is an urgent need to design new vaccines or chemotherapeutics for apicomplexan diseases. High-throughput global protein expression analyses using gel or non-gel based protein separation technologies coupled with mass spectrometry and bioinformatics provide a means to identify new drug and vaccine targets in these pathogens. Protein identification based proteomic projects in apicomplexan parasites is currently underway, with the most significant progress made in the malaria parasite, Plasmodium falciparum. More recently, preliminary two-dimensional gel electrophoresis maps of Toxoplasma gondii and Neospora caninum tachyzoites and Eimeria tenella sporozoites, have been produced, as well as for micronemes in E. tenella. In this review, the status of proteomics in the analysis of global protein expression in apicomplexan parasites will be compared and the challenges associated with these investigations discussed.