3D-printed smartphone-based point of care tool for fluorescence- and magnetophoresis-based cytometry
3D-printed smartphone-based point of care tool for fluorescence- and magnetophoresis-based cytometry
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DOI：
10.1039/c7lc00706j
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发表时间：
2017-08-21
期刊：
影响因子：
6.1
通讯作者：
Tasoglu, Savas
中科院分区：
文献类型：
--
作者：
Knowlton, Stephanie;Joshi, Ashwini;Tasoglu, Savas
在发展中国家，可用于提供重要医疗诊断的资源往往有限，这严重限制了我们诊断病情和进行适当治疗的能力，导致可治疗疾病的死亡率很高。在这里，我们提出了一种多重工具，能够通过磁聚焦与荧光成像并行进行基于密度的细胞分选，以提供高度特异性的临床测定。虽然许多细胞分选技术和荧光显微镜通常成本高昂，并且需要大量的用户培训，限制了发展中国家的可及性和可用性，但该设备结构紧凑、成本低廉且便于携带。该设备可以根据密度分离细胞，从而识别细胞类型和细胞活性，并使用内置智能手机摄像头以明场、暗场或荧光成像模式对细胞进行成像。磁聚焦和荧光成像这两种强大且多功能的技术的结合将使该平台广泛适用于一系列生物医学检测。临床应用包括在资源有限的环境中进行细胞细胞术和基于免疫细胞化学的检测，这最终有助于提高全球医疗诊断的可及性。
In developing countries, there are often limited resources available to provide important medical diagnostics, which severely limits our ability to diagnose conditions and administer proper treatment, leading to high mortality rates for treatable conditions. Here, we propose a multiplex tool capable of density-based cell sorting via magnetic focusing in parallel with fluorescence imaging to provide highly specific clinical assays. While many cell sorting techniques and fluorescence microscopes generally are costly and require extensive user training, limiting accessibility and usability in developing countries, this device is compact, low-cost, and portable. The device can separate cells on the basis of density, which can be used to identify cell type and cell activity, and image the cells in either brightfield, darkfield, or fluorescent imaging modes using the built-in smartphone camera. The combination of these two powerful and versatile techniques magnetic focusing and fluorescence imaging -will make this platform broadly applicable to a range of biomedical assays. Clinical applications include cell cytometry and immunocytochemistry-based assays in limited-resource settings, which can ultimately help to improve worldwide accessibility to medical diagnostics.