Mutation in the P36 gene of Mycobacterium bovis provokes attenuation of the bacillus in a mouse model
Mutation in the P36 gene of Mycobacterium bovis provokes attenuation of the bacillus in a mouse model
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DOI：
10.1016/j.tube.2004.11.002
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发表时间：
2005-07-01
期刊：
影响因子：
3.2
通讯作者：
Cataldi, A
中科院分区：
文献类型：
--
作者：
Bigi, F;Gioffré, A;Cataldi, A
P36是分布于分枝杆菌属的分泌蛋白家族的成员。这些蛋白质的中心结构域包含几个氨基酸PGLTS重复序列，这些重复序列在物种之间差异很大。P36又称输出重复蛋白（exported repeating protein，Erp）。结核分枝杆菌，已被证明与毒力有关，因为其基因的破坏损害了两种毒性M.结核和M.在培养的巨噬细胞和免疫活性小鼠中的牛BCG。为了证明P36是野生型牛分枝杆菌的假定毒力因子，我们通过基因破坏产生了P36突变体，并且我们评估了其在感染小鼠的脾和肺中的复制。在这项研究中，突变株在小鼠中表现出低水平的增殖，表明P36基因对M.牛(c)2004爱思唯尔有限公司保留所有权利。
P36 is a member of a family of secreted proteins distributed throughout the genus Mycobacterium. The central domain of these proteins contains several amino acid PGLTS repeats, which differ considerably between species. P36, also called exported repetitive protein (Erp) in M. tuberculosis, has been shown to be associated with virulence since the disruption of its gene impaired multiplication of both virulent M. tuberculosis and M. bovis BCG in cultured macrophages and immunocompetent mice. In order to demonstrate that P36 is a putative virulence factor of wild-type Mycobacterium bovis we generated a P36 mutant by gene disruption and we evaluated its replication in spleen and lungs of infected mice. In this study, the mutant strain displays low levels of multiplication in mice, indicating that the P36 gene is important for in vivo growth of M. bovis. (c) 2004 Elsevier Ltd. All rights reserved.