Optimization of the Composition of Zwitterionic Copolymers for the Easy-construction of Bio-inactive Surfaces.
Optimization of the Composition of Zwitterionic Copolymers for the Easy-construction of Bio-inactive Surfaces.
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优化两性离子共聚物的组成，以方便构建生物非活性表面。
DOI：
10.1002/jbm.a.35737
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Y.
中科院分区：
文献类型：
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作者：
Nishida;M.; Nakaji-Hirabayashi;T.; Kitano;H.; Matsuoka;K.; Saruwatari;Y.
以2,2′-偶氮二异丁腈为引发剂，在乙醇中合成了两性离子单体羧基甲基甜菜碱（CMB）与小比例的3‐甲基丙烯酰氧基丙基三甲氧基硅烷的无规共聚物（S‐PCMBx）。在共聚物溶液中浸泡后，在玻璃基板上形成了厚度为2-3 nm的S - PCMBxlayer。S - PCMBx修饰的表面具有高度亲水性，抑制了蛋白质（牛血清白蛋白）的非特异性吸附和NIH3T3 -成纤维细胞的粘附。吸附抑制程度随共聚物CMB含量的增加而增加，在CMB含量为90 mol %时达到最大值。相比之下，由于接枝密度低，用末端修饰三甲氧基硅基的PCMB均聚物修饰玻璃基板不能有效抑制蛋白质吸附和细胞粘附。提出了平衡固定点的数量和两性离子聚合物环的长度对于产生生物活性金属氧化物表面的重要性。©2016 Wiley期刊公司[J] .中国生物医学工程学报，2016,35(1):559 - 564。
Random copolymers (S‐PCMBx) of the zwitterionic monomer carboxymethylbetaine (CMB) and a small percentage of 3‐methacryloyloxypropyl trimethoxysilane with various composition ratios were synthesized in ethanol using 2,2′‐azobisisobutyronitrile as the initiator. An S‐PCMBxlayer formed on the glass substrate after soaking in the copolymer solution and had a thickness of 2–3 nm. The S‐PCMBx‐modified surface was highly hydrophilic and suppressed both the non‐specific adsorption of protein (bovine serum albumin) and NIH3T3‐fibroblast adhesion. The degree of adsorption suppression increased with increasing copolymer CMB content with a maximum at 90 mol % CMB. In contrast, the modification of the glass substrate with a PCMB homopolymer terminally modified with a trimethoxysilyl group did not effectively suppress protein adsorption and cell adhesion due to the low graft density. The importance of balancing the number of fixation points and the length of the zwitterionic polymer loops to produce bio‐inactive metal oxide surfaces is suggested. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2029–2036, 2016.