Effect of interferon-π on prostaglandin biosynthesis, transport, and signaling at the time of maternal recognition of pregnancy in cattle:: Evidence of polycrine actions of prostaglandin E2
Effect of interferon-π on prostaglandin biosynthesis, transport, and signaling at the time of maternal recognition of pregnancy in cattle:: Evidence of polycrine actions of prostaglandin E2
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DOI：
10.1210/en.2004-0587
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发表时间：
2004-11-01
期刊：
影响因子：
4.8
通讯作者：
Fortier, MA
中科院分区：
文献类型：
--
作者：
Arosh, JA;Banu, SK;Fortier, MA
妊娠的识别和建立涉及孕体、子宫和黄体（CL）之间的几种分子和细胞相互作用。在反刍动物中，胚胎来源的干扰素-tau（IFNtau）被识别为妊娠识别信号。子宫内膜前列腺素F-2 α（PGF（2 α））是黄体溶解素，而PGE（2）被认为是妊娠建立时的黄体保护或黄体促进介质。IFNtau和子宫内膜PGs的生产，运输和信号转导在母亲识别妊娠（MRP）的时间之间的相互作用还没有得到很好的理解。我们研究了参与PGE（2）和PGF（2 α）代谢的酶、环氧合酶-1（考克斯-1）和考克斯-2、PG脱氢酶（PGES和PGFS）、PG 15-脱氢酶和PG转运蛋白以及PGE（2）（EP 2和EP 3）和PGF（2 α）受体的表达。IFN τ以时空和组织特异性方式影响子宫内膜、子宫肌层和CL中考克斯-2、PGFS、EP 2和EP 3的细胞特异性表达，而它不改变任何这些组织中的考克斯-1、PGES、PG 15-脱氢酶、PG转运蛋白或PGF（2 α）受体表达。在子宫内膜中，IFNtau降低上皮细胞中的PGFS并增加间质中的EP 2。在子宫肌层中，IFNtau降低PGFS并增加平滑肌细胞中的EP 2。在CL中，IFNtau增加PGES并降低EP 3。总之，我们的研究结果表明，IFNtau直接或间接增加MRP过程中子宫内膜、子宫肌层和CL中PGE（2）生物合成和EP 2相关信号。因此，PGE（2）可能在子宫内膜容受性、子宫肌层静止和黄体维持中起关键作用，表明MRP时PGE（2）的多分泌（内分泌、外分泌、旁分泌和自分泌）作用。因此，妊娠的建立可能不仅取决于子宫内膜PGF（2 α）的抑制，而且还取决于牛PGE（2）产量的增加。
Recognition and establishment of pregnancy involve several molecular and cellular interactions among the conceptus, uterus, and corpus luteum (CL). In ruminants, interferon-tau (IFNtau) of embryonic origin is recognized as the pregnancy recognition signal. Endometrial prostaglandin F-2alpha (PGF(2alpha)) is the luteolysin, whereas PGE(2) is considered a luteoprotective or luteotrophic mediator at the time of establishment of pregnancy. The interplay between IFNtau and endometrial PGs production, transport, and signaling at the time of maternal recognition of pregnancy (MRP) is not well understood. We have studied the expression of enzymes involved in metabolism of PGE(2) and PGF(2alpha), cyclooxygenase-1 (COX-1) and COX-2, PG synthases (PGES and PGFS), PG 15-dehydrogenase, and PG transporter as well as PGE(2) (EP2 and EP3) and PGF(2alpha) receptors. IFNtau influences cell-specific expression of COX-2, PGFS, EP2, and EP3 in endometrium, myometrium, and CL in a spatio-temporal and tissue-specific manner, whereas it does not alter COX-1, PGES, PG 15-dehydrogenase, PG transporter, or PGF(2alpha) receptor expression in any of these tissues. In endometrium, IFNtau decreases PGFS in epithelial cells and increases EP2 in stroma. In myometrium, IFNtau decreases PGFS and increases EP2 in smooth muscle cells. In CL, IFNtau increases PGES and decreases EP3. Together, our results show that IFNtau directly or indirectly increases PGE(2) biosynthesis and EP2-associated signaling in endometrium, myometrium, and CL during MRP. Thus, PGE(2) may play pivotal roles in endometrial receptivity, myometrial quiescence, and luteal maintenance, indicating polycrine (endocrine, exocrine, paracrine, and autocrine) actions of PGE(2) at the time of MRP. Therefore, the establishment of pregnancy may depend not only on inhibition of endometrial PGF(2alpha), but also on increased PGE(2) production in cattle.