Stromal cell-derived receptor 2 and cytochrome b561 are functional ferric reductases.
Stromal cell-derived receptor 2 and cytochrome b561 are functional ferric reductases.
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DOI：
10.1016/s1570-9639(03)00242-5
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发表时间：
2003-09
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影响因子：
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通讯作者：
J. D. Vargas;B. Herpers;A. Mckie;S. Gledhill;J. McDonnell;M. van den Heuvel;K. Davies;C. Ponting
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文献类型：
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作者：
J. D. Vargas;B. Herpers;A. Mckie;S. Gledhill;J. McDonnell;M. van den Heuvel;K. Davies;C. Ponting
铁在细胞生物中具有多种功能，从电子传递和DNA合成到三磷酸腺苷（ATP）和神经递质合成。铁的体内平衡失调会导致认知障碍和脱髓鞘障碍，而铁过量则会导致神经退行性疾病。在这项研究中，我们发现预测的铁还原酶b561家族的三个成员，即小鼠细胞色素b561和小鼠和苍蝇基质细胞衍生受体2 (SDR2)，具有铁还原酶活性。鉴于第四个成员，十二指肠细胞色素b (Dcytb)，先前已被证明是一种铁还原酶，很可能该家族的所有其他成员也表现出这种活性。此外，我们发现大鼠的sdr2信息主要在肝脏和肾脏中表达，在十二指肠中表达较少。在低转铁酶血症（hpx）小鼠中，sdr2在肝脏和肾脏中的表达降低，提示其可能受铁的调节。此外，通过原位杂交分析，我们证明小鼠sdr2存在于脉络膜丛和四个脑室的室管膜细胞中。
Iron has a variety of functions in cellular organisms ranging from electron transport and DNA synthesis to adenosine triphosphate (ATP) and neurotransmitter synthesis. Failure to regulate the homeostasis of iron can lead to cognition and demyelination disorders when iron levels are deficient, and to neurodegenerative disorders when iron is in excess. In this study we show that three members of the b561 family of predicted ferric reductases, namely mouse cytochrome b561 and mouse and fly stromal cell-derived receptor 2 (SDR2), have ferric reductase activity. Given that a fourth member, duodenal cytochrome b (Dcytb), has previously been shown to be a ferric reductase, it is likely that all remaining members of this family also exhibit this activity. Furthermore, we show that the rat sdr2 message is predominantly expressed in the liver and kidney, with low expression in the duodenum. In hypotransferrinaemic (hpx) mice, sdr2 expression in the liver and kidney is reduced, suggesting that it may be regulated by iron. Moreover, we demonstrate the presence of mouse sdr2 in the choroid plexus and in the ependymal cells lining the four ventricles, through in situ hybridization analysis.