DEGRADATION OF BLOOD-GROUP ANTIGENS IN HUMAN COLON ECOSYSTEMS .1. INVITRO PRODUCTION OF ABH BLOOD GROUP DEGRADING ENZYMES BY ENTERIC BACTERIA
DEGRADATION OF BLOOD-GROUP ANTIGENS IN HUMAN COLON ECOSYSTEMS .1. INVITRO PRODUCTION OF ABH BLOOD GROUP DEGRADING ENZYMES BY ENTERIC BACTERIA
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DOI：
10.1172/jci108270
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发表时间：
1976-01-01
影响因子：
15.9
通讯作者：
BOULDING, ET
中科院分区：
文献类型：
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作者：
HOSKINS, LC;BOULDING, ET
人类粪便中含有由肠道细菌产生的酶，这些酶可降解肠粘蛋白糖蛋白的A、B和H血型抗原。我们研究了它们在粪便培养物中的产生，以确定这种培养物是否可以成为酶纯化的来源，并探索血型抗原降解酶如何适应个体人类结肠生态系统。它们存在于27名健康受试者的粪便培养物中，包括ABH非分泌型受试者。热敏感的专性厌氧菌是其主要来源。从39%到85%的总酶活性产生的增长文化是胞外的。商业猪胃粘蛋白和唾液糖蛋白，包括缺乏A、B和H抗原的莱亚唾液，增强厌氧粪便培养物中A-、B-和H-降解活性的产生，而与糖蛋白的血型特异性无关。有证据表明，宿主的ABO血型和分泌状态会影响其粪便细菌在体外产生的血型降解酶的特异性。因此，粪便接种物从B分泌与猪胃粘蛋白（A和H特异性）或与莱亚唾液孵育产生更高水平的B-降解比A-或H-降解活性，和接种物从A分泌在类似的媒体产生更高水平的A-降解比B-或H-降解活性。粪便培养物中产生的血型降解酶是糖苷酶而不是蛋白酶。B-降解酶以B特异性从粘蛋白糖蛋白的寡糖侧链上切割B抗原决定簇α-D-半乳糖。含有血型物质的厌氧粪便培养物是纯化血型抗原降解酶的可行来源。在A型和B型分泌型肠粘蛋白中预先适应血型抗原会影响体外产生的酶的特异性。
Human feces contain enzymes produced by enteric bacteria that degrade the A, B, and H blood group antigens of gut mucin glycoproteins. We have studied their production in fecal cultures to determine if such cultures can be a source for enzyme purification and to explore how blood group antigen-degrading enzymes are adapted in individual human colon ecosystems. They were present in fecal cultures from each of 27 healthy subjects, including ABH nonsecretors. Heat-sensitive obligate anaerobes are their major source. From 39 to 85% of the total enzyme activity produced by growing cultures was extracellular. Commercial hog gastric mucin and salivary glycoproteins, including Lea saliva which lacks A, B, and H antigens, enhance production of A-, B-, and H-degrading activity in anaerobic fecal cultures irrespective of the glycoprotein's blood group specificity. There is evidence that the host's ABO blood type and secretor status affects the specificity of blood group-degrading enzymes produced by his fecal bacteria in vitro. Thus, fecal inocula from B secretors incubated with hog gastric mucin (A and H specificity) or with Lea saliva produced greater levels of B-degrading than A- or H-degrading activity, and inocula from A secretors in similar media produced greater levels of A-degrading than B- or H-degrading activity. Blood group-degrading enzymes produced in fecal cultures are glycosidases and not proteases. The B-degrading enzyme cleaves the B antigenic determinant alpha-D-galactose from the oligosaccharide side chains of mucin glycoproteins with B specificity. Anaerobic fecal cultures containing blood group substances are a feasible source for purifying blood group antigen-degrading enzymes. Prior adaptation to blood group antigens in the gut mucins of type A and type B secretors affects the specificity of the enzymes produced in vitro.