CLONING AND DEVELOPMENTAL EXPRESSION OF THE CHICK TYPE-II AND TYPE-III TGF-BETA RECEPTORS
CLONING AND DEVELOPMENTAL EXPRESSION OF THE CHICK TYPE-II AND TYPE-III TGF-BETA RECEPTORS
复制标题
DOI：
10.1002/aja.1001990103
复制
发表时间：
1994-01-01
影响因子：
2.5
通讯作者：
MAAS, RL
中科院分区：
文献类型：
--
作者：
BARNETT, JV;MOUSTAKAS, A;MAAS, RL
为了阐明多肽生长因子在鸡器官发生中的作用，我们重点研究了转化生长因子β2，并克隆了鸡的II型和III型转化生长因子β受体。鸡II型受体是一种丝氨酸/苏氨酸激酶，与人类受体具有相同的配基结合特征，而与哺乳动物受体相比则具有不同的N末端。鸡III型受体是一种β-多糖，其结合特性与大鼠受体相同，并且含有一个跨膜结构域和短细胞质尾巴，与哺乳动物受体相比高度保守。在鸡胚胎发育期间，II型和III型转化生长因子β受体在发育中的心脏、肺和眼睛中共同表达，并在心脏和肺中平行表达。在不同发育阶段培养的心肌细胞中，受体蛋白和mRNAs的水平也会增加，这与在发育中的心脏中观察到的II型和III型受体mRNA水平的增加是一致的。尽管表现出与受体不同的时间或空间分布，但转化生长因子β2也在发育中的心、肺和眼睛中表达。这些发现与最近的数据一致，这些数据表明，II型和III型转化生长因子β受体的高亲和力结合需要II型和III型转化生长因子β受体的共同表达，提示转化生长因子β2和II型和III型TG；FP受体参与了心、肺和眼睛的发育。(C)1994年Wiley-Liss，Inc.
To address the role of peptide growth factors in chick organogenesis, we have focused on TGF beta 2 and have cloned the chick Type II and Type III TGF beta receptors. The chick Type II receptor is a serine/threonine kinase with a ligand binding profile identical to the human receptor and a divergent N-terminus when compared to the mammalian receptors. The chick Type III receptor is a betaglycan that demonstrates a binding profile identical to the rat receptor and contains a single transmembrane spanning domain and short cytoplasmic tail that are highly conserved when compared to the mammalian receptors. Both the Type II and Type III TGF beta receptors are coexpressed during chick embryogenesis in the developing heart, lung, and eye, and are developmentally upregulated in parallel in the heart and lung. Levels of both receptor proteins and mRNAs also increase in cardiocytes cultured from different developmental stages, in agreement with the increase in Type II and Type III receptor mRNA levels observed in the developing heart. Although exhibiting different temporal or spatial profiles from the receptors, TGF beta 2 is also expressed in the developing heart, lung, and eye. These findings are consistent with recent data indicating that coexpression of both the Type II and Type III TGF beta receptors is required for high affinity binding of TGF beta 2 by the Type II receptor and suggest that TGF beta 2 and the Type II and Type III TG;FP receptors participate in heart, lung, and eye development. (C) 1994 Wiley-Liss, Inc.