3D Printing of Neural Tissues Derived from Human Induced Pluripotent Stem Cells Using a Fibrin-Based Bioink
3D Printing of Neural Tissues Derived from Human Induced Pluripotent Stem Cells Using a Fibrin-Based Bioink
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DOI：
10.1021/acsbiomaterials.8b01235
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发表时间：
2019-01-01
影响因子：
5.8
通讯作者：
Willerth, Stephanie M.
中科院分区：
文献类型：
--
作者：
Abelseth, Emily;Abelseth, Laila;Willerth, Stephanie M.
3D生物打印提供了组织工程过程自动化的机会，该过程将生物材料支架和细胞结合起来，为患病或受损组织生成替代品。这些生物打印方法通过将包封在生物墨水中的细胞定位到所得构建体中的特定位置来构建组织替代物。人类诱导多能干细胞（hiPSC）在工程化神经组织时充当重要工具。这些细胞可以无限扩增并分化成中枢神经系统中发现的细胞类型，包括神经元。用于使hiPSC分化成神经组织的一种常见方法需要在化学成分确定的培养基中培养的限定直径的微孔内形成聚集体。然而，这种hiPSC衍生的聚集体的3D生物打印以前在文献中尚未报道，因为它需要开发专门的生物墨水来支持细胞存活和分化成成熟的神经表型。在这里，我们详细介绍了方法，包括制备生物墨水的基础材料成分，生产生物墨水，以及使用Aspect Biosystems的新型RX1打印机和他们的打印机实验室（LOP）技术打印来自hiPSC衍生神经聚集体的3D神经组织的步骤。
3D bioprinting offers the opportunity to automate the process of tissue engineering, which combines biomaterial scaffolds and cells to generate substitutes for diseased or damaged tissues. These bioprinting methods construct tissue replacements by positioning cells encapsulated in bioinks into specific locations in the resulting constructs. Human induced pluripotent stem cells (hiPSCs) serve as an important tool when engineering neural tissues. These cells can be expanded indefinitely and differentiated into the cell types found in the central nervous systems, including neurons. One common method for differentiating hiPSCs into neural tissue requires the formation of aggregates inside of defined diameter microwells cultured in chemically defined media. However, 3D bioprinting of such hiPSC-derived aggregates has not been previously reported in the literature, as it requires the development of specialized bioinks for supporting cell survival and differentiation into mature neural phenotypes. Here we detail methods including preparing base material components of the bioink, producing the bioink, and the steps involved in printing 3D neural tissues derived from hiPSC-derived neural aggregates using Aspect Biosystems' novel RX1 printer and their lab-on-a printer (LOP) technology.