Dictyostelium transcriptional host cell response upon infection with Legionella
Dictyostelium transcriptional host cell response upon infection with Legionella
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DOI：
10.1111/j.1462-5822.2005.00633.x
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发表时间：
2006-03-01
影响因子：
3.4
通讯作者：
Eichinger, L
中科院分区：
文献类型：
--
作者：
Farbrother, P;Wagner, C;Eichinger, L
利用DNA微阵列技术研究了盘基网柄菌感染嗜肺军团菌后的差异基因表达。对48小时感染过程的研究表明，有几个共调控基因簇，在不同的功能类别中优先上调或下调基因，并显示大多数转录变化发生在感染后24小时。对感染后24 h的时间点进行了详细的分析，并与三个对照组、未感染的细胞以及与哈克氏军团菌和嗜肺乳杆菌共孵育进行了比较。131个差异表达的盘状芽孢杆菌基因被鉴定为所有三个实验的共同基因，并被认为参与了致病反应。对差异调控基因的功能注释表明，军团菌除了触发应激反应外，显然不仅干扰细胞内小泡的融合和去向，而且还深刻地影响和利用其宿主的新陈代谢。对于一些已识别的基因，例如最近的一项研究证明rtoA参与了宿主的反应，对于其他一些基因来说，这种作用似乎是可信的。这些结果为更好地理解复杂的宿主-病原体相互作用以及进一步研究网柄基菌对军团菌感染的反应提供了基础。
Differential gene expression of Dictyostelium discoideum after infection with Legionella pneumophila was investigated using DNA microarrays. Investigation of a 48 h time course of infection revealed several clusters of co-regulated genes, an enrichment of preferentially up- or downregulated genes in distinct functional categories and also showed that most of the transcriptional changes occurred 24 h after infection. A detailed analysis of the 24 h time point post infection was performed in comparison to three controls, uninfected cells and co-incubation with Legionella hackeliae and L. pneumophila Delta dotA. One hundred and thirty-one differentially expressed D. discoideum genes were identified as common to all three experiments and are thought to be involved in the pathogenic response. Functional annotation of the differentially regulated genes revealed that apart from triggering a stress response Legionella apparently not only interferes with intracellular vesicle fusion and destination but also profoundly influences and exploits the metabolism of its host. For some of the identified genes, e.g. rtoA involvement in the host response has been demonstrated in a recent study, for others such a role appears plausible. The results provide the basis for a better understanding of the complex host-pathogen interactions and for further studies on the Dictyostelium response to Legionella infection.