Molecular identity of the outwardly rectifying, swelling-activated anion channel: time to reevaluate pICln.
Molecular identity of the outwardly rectifying, swelling-activated anion channel: time to reevaluate pICln.
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向外整流、膨胀激活的阴离子通道的分子特性：重新评估 pICln 的时间。
DOI：
10.1085/jgp.111.5.617
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Strange,K
中科院分区：
文献类型：
--
作者：
Strange,K
细胞通过激活阴离子和阳离子通道来响应肿胀，这些通道允许无机离子和有机溶质的被动损失。净溶质流出伴随着强制水的功能，以返回细胞体积向其原始值，一个过程称为regulatory volume reduction.An明显普遍存在的脊椎动物细胞肿胀的反应是激活一个向外整流阴离子电流称为ICl。好极了。该电流的一般特征包括Eisenman I型阴离子渗透性序列（I Br Cl F）、适度的向外整流、在高于ECl的电位下的电压依赖性失活、由包括常规阴离子转运抑制剂在内的多种化合物的抑制以及由细胞外核苷酸如ATP的阻断（由Strange等人，1996; Okada，1997）。整流程度、电压敏感性和药理学在不同的细胞类型之间可能有所不同。目前尚不清楚观察到的差异是否反映了不同通道的存在，或者它们是否是由于实验和/或生理变量。例如，通道电压敏感性受细胞内Mg 2浓度（Okada，1997）、跨膜Cl梯度和细胞外溶液中存在的外源阴离子（Meyer和Korbmacher，1996; Okada，1997;我未发表的观察结果）的影响。在本文中，为了简单起见，我将假设ICl。膨胀由单个通道类型或一系列密切相关的通道的活动引起。负责ICl的通道。膨胀有多种名称，包括VRAC（体积调节阴离子通道）和VSOR（体积膨胀感应向外整流阴离子通道）。该通道的另一个名称是VSOAC（体积敏感性有机渗压剂/阴离子通道），其被创造以反映其在有机阴离子和电中性有机渗压剂的运输中所起的假定作用（Strange等人，1996; Kirk and Strange，1998）.为了避免混淆，我将在本文中将该通道简称为ICl。涌浪通道
Cells respond to swelling by activating anion and cation channels that allow the passive loss of inorganic ions and organic solutes. Net solute efflux accompanied by osmotically obliged water functions to return cell volume towards its original value, a process termed regulatory volume decrease.An apparently ubiquitous response to swelling in vertebrate cells is activation of an outwardly rectifying anion current termed ICl. swell. The general characteristics of this current include an Eisenman type I anion permeability sequence (I Br Cl F), modest outward rectification, voltage-dependent inactivation at potentials above ECl, inhibition by a wide variety of compounds, including conventional anion transport inhibitors, and block by extracellular nucleotides such as ATP (reviewed by Strange et al., 1996; Okada, 1997). The degree of rectification, voltage sensitivity, and pharmacology can vary somewhat between different cell types. It is not clear whether the differences observed reflect the existence of distinct channels, or whether they are due to experimental and/or physiological variables. For example, channel voltage sensitivity is altered by intracellular Mg2 concentration (Okada, 1997), the transmembrane Cl gradient, and by the presence of foreign anions in the extracellular solution (Meyer and Korbmacher, 1996; Okada, 1997; my unpublished observations). In this article, I will assume for the sake of simplicity that ICl. swell arises from the activity of a single channel type or a family of closely related channels. The channel responsible for ICl. swell has been referred to by a variety of names, including VRAC (volume regulated anion channel) and VSOR (volume expansionsensing outwardly rectifying anion channel). Another name for the channel is VSOAC (volume-sensitive organic osmolyte/anion channel), which was coined to reflect the putative role it plays in the transport of organic anions and electroneutral organic osmolytes (Strange et al., 1996; Kirk and Strange, 1998). To avoid confusion, I will refer to the channel in this paper simply as the ICl. swell channel.
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人类年轻红细胞中氯离子通道相关蛋白 pICln 的分子克隆和表达：与肌动蛋白的关联。
DOI：
10.1042/bj3270609
发表时间：
1997
期刊：
The Biochemical journal
影响因子：
--
作者：
Schwartz,RS;Rybicki,AC;Nagel,RL
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影响因子：
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作者：
M. Paulmichl;Yi Li;K. Wickman;M. Ackerman;E. Peralta;D. Clapham
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DOI：
10.1146/annurev.physiol.60.1.719
发表时间：
1998
期刊：
Annual review of physiology.
影响因子：
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作者：
Kirk,K;Strange,K
通讯作者：
Strange,K
影响因子：
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作者：
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细胞肿胀对 pICln 膜和细胞质分布的影响。
DOI：
10.1152/ajpcell.1998.274.6.c1545
发表时间：
1998
期刊：
The American journal of physiology
影响因子：
--
作者：
Emma,F;Breton,S;Morrison,R;Wright,S;Strange,K
通讯作者：
Strange,K