Low Counts of Faecalibacterium prausnitzii in Colitis Microbiota
Low Counts of Faecalibacterium prausnitzii in Colitis Microbiota
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DOI：
10.1002/ibd.20903
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发表时间：
2009-08-01
影响因子：
4.9
通讯作者：
Dore, J.
中科院分区：
文献类型：
--
作者：
Sokol, H.;Seksik, P.;Dore, J.
背景：肠道微生物群被怀疑在结肠炎，尤其是炎症性肠病（IBD）的发病机制中起作用。目的是比较结肠炎患者与健康受试者（HS）的粪便微生物群组成。
方法：对222例活动性克罗恩病（A - CD）患者、10例缓解期克罗恩病（R - CD）患者、13例活动性溃疡性结肠炎（A - UC）患者、4例缓解期溃疡性结肠炎（R - UC）患者、8例感染性结肠炎（IC）患者以及27例健康受试者的粪便样本，采用针对16S rRNA基因的实时定量聚合酶链反应（PCR）进行分析。将细菌计数转换为对数（Log(10)CFU）进行统计分析。
结果：厚壁菌门（柔嫩梭菌和球形梭菌组）的细菌在活动性IBD患者（9.7；P = 0.004）和感染性结肠炎患者（9.4；P = 0.02）中的含量低于健康受试者（10.8）。普拉梭菌（柔嫩梭菌组的一个主要代表菌种）在活动性IBD患者和感染性结肠炎患者中的数量低于健康受试者（分别为8.8和8.3对比10.4；P = 0.0004和P = 0.003）。厚壁菌门/拟杆菌门的比值在活动性IBD患者（1.3；P = 0.0001）和感染性结肠炎患者（0.4；P = 0.002）中较低。与健康受试者相比，双歧杆菌在活动性IBD患者和感染性结肠炎患者中的含量较低（分别为7.9和7.7对比9.2；P = 0.001和P = 0.01）。
结论：IBD患者的粪便微生物群与健康受试者不同。厚壁菌门，尤其是普拉梭菌，在活动性IBD患者以及感染性结肠炎患者中含量不足。这些细菌对肠道内稳态可能至关重要，因为普拉梭菌数量减少始终与肠道黏膜保护作用降低相关。
Background: The intestinal microbiota is Suspected to play a role ill colitis and particularly ill inflammatory bowel disease (IBD) pathogenesis. The aim was, to compare the fecal microbiota composition of patients with colitis to that of healthy subjects (HS).Methods: fecal samples front 222 active Crohn's disease (A-CD) patients, 10 CD patients in remission (R-CD), 13 active ulcerative colitis (A-UC) patients. 4 UC patients in remission (R-UC), 8 infectious colitis (IC) patients, and 27 HS were analyzed by quantitative real-time polymerase chain reaction (PCR) targeting the 16S rRNA gene. Bacterial counts were transformed to logarithms (Log(10) CFU) for statistical analysis.Results: Bacteria of the phylum Firmicutes (Clostridium leptum and Clostridium coccoides groups) were less represented in A-IBD patients (9.7; P = 0.004) and IC (9.4; P = 0.02). compared to HS (10.8), Faecalibacterium prausnitzii species (a major representative of the C. leptum group) had lower Counts in A-IBD and IC patients compared to HS (8.8 and 8.3 versus 10.4; P = 0.0004 and P = 0.003) The Firmicutes/Bacteroidetes ratio was lower in A-IBD (1.3; P = 0.0001) and IC patients (0.4; P = 0.002). Compared to HS, Bifidobacteria were less represented in A-IBD and IC (7.9 and 7.7 Versus 9.2; P = 0.001 and P = 0.01).Conclusions: The fecal microbiota of patients with IBD differs front that of HS. The phylum Firmicutes and particularly the species F. prausnitzii, are underrepresented in A-IBD patients as well as in IC patients. These bacteria could be crucial to Out homeostasis since lower counts Of F. prausnitzii are consistently associated with a reduced protection of the gut mucosa.