Tubulization with chitosan guides for the repair of long gap peripheral nerve injury in the rat
Tubulization with chitosan guides for the repair of long gap peripheral nerve injury in the rat
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DOI：
10.1002/micr.22362
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发表时间：
2015-05-01
期刊：
影响因子：
2.1
通讯作者：
Navarro, X.
中科院分区：
文献类型：
--
作者：
Gonzalez-Perez, F.;Cobianchi, S.;Navarro, X.
生物合成引导物可以替代神经移植物用于重建严重损伤的周围神经。本研究的目的是评估再生能力的壳聚糖管桥接关键的神经间隙（15 mm长）在大鼠坐骨神经相比，硅树脂（SIL）管和神经自体移植物（AGs）。将28只Wistar汉诺威大鼠随机分为4组（n = 7），分别用SIL管、低乙酰化度（约2%，DAI）和中乙酰化度（约5%，DAII）的壳聚糖导管和AG修复神经。沿着4个月，连续进行电生理和痛觉测试，并在研究结束时进行组织形态学分析。植入4个月后，两组壳聚糖管的功能恢复程度相似，管中部有髓神经纤维数量相似。壳聚糖管的结果明显优于SIL管（P < 0.01），但低于AG管（P < 0.01）。与AG相反，所有大鼠都有有效的再生和靶神经再支配，DAI和DAII的壳聚糖管分别获得了43%和57%的成功率，而SIL管修复的所有动物的再生均失败。本研究表明，壳聚糖导管是一种很有前途的管道，构建人工神经移植物。(c)2014 Wiley Periodicals，Inc.显微外科35：300-308，2015年。
Biosynthetic guides can be an alternative to nerve grafts for reconstructing severely injured peripheral nerves. The aim of this study was to evaluate the regenerative capability of chitosan tubes to bridge critical nerve gaps (15 mm long) in the rat sciatic nerve compared with silicone (SIL) tubes and nerve autografts (AGs). A total of 28 Wistar Hannover rats were randomly distributed into four groups (n = 7 each), in which the nerve was repaired by SIL tube, chitosan guides of low (approximate to 2%, DAI) and medium (approximate to 5%, DAII) degree of acetylation, and AG. Electrophysiological and algesimetry tests were performed serially along 4 months follow-up, and histomorphometric analysis was performed at the end of the study. Both groups with chitosan tubes showed similar degree of functional recovery, and similar number of myelinated nerve fibers at mid tube after 4 months of implantation. The results with chitosan tubes were significantly better compared to SIL tubes (P < 0.01), but lower than with AG (P < 0.01). In contrast to AG, in which all the rats had effective regeneration and target reinnervation, chitosan tubes from DAI and DAII achieved 43 and 57% success, respectively, whereas regeneration failed in all the animals repaired with SIL tubes. This study suggests that chitosan guides are promising conduits to construct artificial nerve grafts. (c) 2014 Wiley Periodicals, Inc. Microsurgery 35:300-308, 2015.