Comparison of picornaviral IRES-driven internal initiation of translation in cultured cells of different origins
Comparison of picornaviral IRES-driven internal initiation of translation in cultured cells of different origins
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DOI：
10.1093/nar/25.5.925
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发表时间：
1997-03-01
影响因子：
14.9
通讯作者：
Kean, KM
中科院分区：
文献类型：
--
作者：
Borman, AM;LeMercier, P;Kean, KM
我们最近比较了6个小核糖核酸病毒内部核糖体进入片段（IRES）和丙型肝炎病毒（HCV）IRES驱动体外翻译内部起始的能力。在这里，我们提出了一个类似的比较结果，在六个不同的培养细胞系感染的重组牛痘病毒表达的T7聚合酶和双顺反子质粒转染。IRES可以分为三组：（i）心脏病毒和口疮病毒IRES（和HCV元件）在所有测试的细胞系中有效地引导内部起始;（ii）肠道病毒和鼻病毒IRES在几种细胞系中至少同样有效，但在某些细胞类型中效率极低;和（iii）甲型肝炎病毒IRES不能在所用的任何细胞系（包括）中有效地引导内部起始。人肝细胞）。当IRES根据其体外活性或序列同源性进行分类时，发现这三个组是相同的。在小鼠神经元细胞系中，脊髓灰质炎病毒和其他I型IRES在人工双顺反子环境中不起作用。然而，在用基因组长度的RNA转染这些细胞后，有效地产生了感染性脊髓灰质炎病毒。此外，I型IRES的活性在脊髓灰质炎病毒2A蛋白酶的共表达后显著增加，表明虽然IRES效率可能在不同细胞类型之间有很大差异，但至少在某些情况下，病毒蛋白能够克服细胞特异性翻译缺陷。
We recently compared the efficiency of six picornaviral internal ribosome entry segments (IRESes) and the hepatitis C virus (HCV) IRES for their ability to drive internal initiation of translation invitro. Here we present the results of a similar comparison performed in six different cultured cell lines infected with a recombinant vaccinia virus expressing the T7 polymerase and transfected with dicistronic plasmids. The IRESes could be divided into three groups:(i) the cardiovirus and aphthovirus IRESes (and the HCV element) direct internal initiation efficiently in all cell lines tested; (ii) the enterovirus and rhinovirus IRESes are at least equally efficient in several cell lines, but are extremely inefficient in certain cell types; and (iii) the hepatitis A virus IRES is incapable of directing efficient internal initiation in any of the cell lines used (including. human hepatocytes). These are the same three groups found when IRESes were classified according to their activities in vitro, or according to sequence homologies. In a mouse neuronal cell line, the poliovirus and other type I IRESes were not functional in an artificial bicistronic context. However, infectious poliovirions were produced efficiently after transfection of these cells with a genomic length RNA. Furthermore, activity of the type I IRESes was dramatically increased upon co-expression of the poliovirus 2A proteinase, demonstrating that while IRES efficiency may vary considerably from one cell type to another, at least in some cases viral proteins are capable of overcoming cell-specific translational defects.