Ca ion uptake by rat kidney mitochondria and its dependence on respiration and phosphorylation.
Ca ion uptake by rat kidney mitochondria and its dependence on respiration and phosphorylation.
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DOI：
10.1016/s0021-9258(19)73805-8
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发表时间：
1962-08
期刊：
影响因子：
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通讯作者：
F. D. Vasington;J. V. Murphy
中科院分区：
文献类型：
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作者：
F. D. Vasington;J. V. Murphy
结果：大鼠肾线粒体对Ca++结合的要求-表1数据表明，大鼠肾线粒体可以从悬浮介质中吸收大量Ca++。当培养基中含有呼吸底物、ATP、Pi和Mg ' +时，ca2 +结合量最大。当从最优培养基中省略ATP时，没有大量的Ca++结合（表1，实验2），而当省略Pi时，Ca++的吸收量从40%下降到70%。Pi浓度对ca2 ++结合的影响如图1所示；大约4my Pi支持最大Ca++吸收。图2中的数据显示了STP浓度的影响；最大的Ca++结合需要大约3 rnM的ATP。
RESULTSRequirements for Ca++ Binding bg Xitochondria-Data shown in Table I indicate that rat kidney mitochondria can take up large amounts of Ca++ from the suspending medium. The amount of Ca++ bound was maximal when the medium contained a respiratory substrate, ATP, Pi, and Mg’+. When ATP was omitted from the optimal medium, no significant amount of Ca++ was bound (Table I, Experiment 2), whereas when Pi was omitted, the amount of Ca++ taken up was decreased from 40 to70%. The effect of Pi concentration on Ca++ binding is shown in Fig. 1; approximately 4 my Pi supported maximal Ca++ uptake. The data in Fig. 2 show the effect of STP concentration; approximately 3 rnM ATP was required for maximal Ca++ binding.