PHA production from the organic fraction of municipal solid waste (OFMSW): Overcoming the inhibitory matrix
PHA production from the organic fraction of municipal solid waste (OFMSW): Overcoming the inhibitory matrix
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DOI：
10.1016/j.watres.2016.03.033
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发表时间：
2016-06-01
期刊：
影响因子：
12.8
通讯作者：
Kleerebezem, Robbert
中科院分区：
文献类型：
--
作者：
Korkakaki, Emmanouela;Mulders, Michel;Kleerebezem, Robbert
从城市固体废物（OFMSW）的源分离的有机部分的渗滤液作为聚羟基脂肪酸酯（PHA）的生产的基板进行了评价。最初，富集步骤是直接进行的渗滤液中的盛宴-饥荒制度。富集的生物质的细胞PHA含量的最大化产生低PHA含量（29重量%），表明PHA生产者的选择是不成功的。当基板的富集被切换到一个合成的挥发性脂肪酸（VFA）的混合物-类似的VFA碳组成的渗滤液-PHA生产商获得了竞争优势和主导地位。随后的积累与渗滤液中的营养过剩的条件下，导致在78重量%的最大PHA含量。基于实验结果，在“干净的”VFA流中富集PHA生产群落，然后从不允许富集但确实能够实现高细胞PHA含量的流（例如OFMSW浸出液）中积累PHA，使得整个过程在经济上更具吸引力。与直接使用复杂基质进行富集和积累相比，估计的总工艺产率可提高四倍。(C)2016爱思唯尔有限公司版权所有
Leachate from the source separated organic fraction of municipal solid waste (OFMSW) was evaluated as a substrate for polyhydroxyalkanoates (PHA) production. Initially, the enrichment step was conducted directly on leachate in a feast-famine regime. Maximization of the cellular PHA content of the enriched biomass yielded to low PHA content (29 wt%), suggesting that the selection for PHA-producers was unsuccessful. When the substrate for the enrichment was switched to a synthetic volatile fatty acid (VFA) mixture-resembling the VFA carbon composition of the leachate-the PHA-producers gained the competitive advantage and dominated. Subsequent accumulation with leachate in nutrient excess conditions resulted in a maximum PHA content of 78 wt%. Based on the experimental results, enriching a PHA-producing community in a "clean" VFA stream, and then accumulating PHA from a stream that does not allow for enrichment but does enable a high cellular PHA content, such as OFMSW leachate, makes the overall process much more economically attractive. The estimated overall process yield can be increased four-fold, in comparison to direct use of the complex matrix for both enrichment and accumulation. (C) 2016 Elsevier Ltd. All rights reserved.