Prussian Blue Dispersed Sphere Catalytic Labels for Amplified Electronic Detection of DNA.
Prussian Blue Dispersed Sphere Catalytic Labels for Amplified Electronic Detection of DNA.
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DOI：
10.1002/elan.200704063
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发表时间：
2008-02
期刊：
影响因子：
3
通讯作者：
Siriwan Suwansa-ard;Yun Xiang;R. Bash;P. Thavarungkul;P. Kanatharana;Joseph Wang
中科院分区：
文献类型：
--
作者：
Siriwan Suwansa-ard;Yun Xiang;R. Bash;P. Thavarungkul;P. Kanatharana;Joseph Wang
本文报道了一种以普鲁士蓝（PB）修饰的聚合物微球作为寡核苷酸标记物的高灵敏电化学DNA杂交分析方法。夹心检测依赖于二级核酸探针，其用载有许多普鲁士蓝纳米颗粒的聚苯乙烯珠官能化。捕获的PB“人工过氧化物酶”标签对过氧化氢还原的非常强的催化活性，沿着许多催化颗粒包封在聚合物珠上，允许对低至50 fM水平（2.5阿莫尔）的DNA靶进行安培检测。成像和光谱测量用于表征PB标记的聚苯乙烯珠。PB催化标记与聚合物载体珠的这种偶联为不同生物分子相互作用的放大转导提供了巨大的希望。
A highly sensitive electrochemical DNA hybridization assay using Prussian blue (PB)-modified polymeric spheres as the oligonculeotide labeling tag is described. The sandwich assay relies on a secondary nucleic-acid probe functionalized with polystyrene beads loaded with numerous Prussian blue nanoparticles. The very strong catalytic activity of the captured PB 'artificial peroxidase' tag towards the reduction of hydrogen peroxide, along with the encapsulation of numerous catalytic particles onto polymeric beads, allows amperometric detection of the DNA target down to the 50 fM level (2.5 amol). Imaging and spectroscopic measurements are used to characterize the PB-tagged polystyrene beads. Such coupling of PB catalytic labels with polymeric carrier beads offers great promise for amplified transduction of different biomolecular interactions.