Implication of expression of Nanog in prostate cancer cells and their stem cells
Implication of expression of Nanog in prostate cancer cells and their stem cells
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DOI：
10.1007/s11596-012-0043-5
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发表时间：
2012-04-01
影响因子：
--
通讯作者：
Qi, Jun
中科院分区：
文献类型：
--
作者：
Gong, Chen;Liao, Hui;Qi, Jun
最近的研究表明，前列腺癌可能是由前列腺癌干细胞引起的，这些干细胞与正常干细胞具有一些相同的特征。本研究旨在检测Nanog在PC 3前列腺癌细胞系及其肿瘤干细胞中的表达差异，初步探讨Nanog与前列腺癌及其肿瘤干细胞的关系。利用磁活性细胞分选（MACS）技术，从PC 3细胞系中分离出一群具有干细胞特征的CD 44（+）/CD 133（+）前列腺癌细胞。免疫组化结果显示，分离的细胞表达CD 44、CD 133和α 2 β 1整合素（integin）。CCK-8分析显示分离的细胞具有较强的增殖能力。在无血清培养液中形成细胞球，在有血清培养液中形成全克隆，表明分离的细胞具有自我更新的能力，表明分离的细胞是来源于PC 3细胞系的癌干细胞样细胞群。Western blotting显示，分离的细胞中胚胎干细胞标记物Nanog的表达高于PC 3细胞。我们的研究表明Nanog可能有助于维持前列腺癌干细胞的自我更新和未分化，并可能作为前列腺癌干细胞分离和鉴定的标志物。
Recent studies suggested that the prostate cancer may arise from prostate cancer stem cells that share some same characteristics with normal stem cells. The purpose of this study was to detect the differences of Nanog expression between PC3 prostate cancer cell line and its tumor stem cells, and the relationship was preliminarily examined between Nanog and prostate cancer and its tumor stem cells. By using magnetic active cell sorting (MACS), we isolated a population of CD44(+)/CD133(+) prostate cancer cells that display stem cell characteristics from PC3 cell line. Immunohistochemistry revealed positive expressions of CD44, CD133 and alpha(2)beta(1)-integin in the isolated cells. CCK-8 analysis showed that isolated cells had a strong proliferative ability. The formation of the cell spheres in serum-free medium and holoclones in serum-supplied medium showed that the cells were capable of self-renewing, indicating that the isolated cells were a population of cancer stem-like cells derived from PC3 cell line. Western blotting exhibited that the isolated cells had higher experession of Nanog, an embryonic stem marker, as compared with PC3 cells. Our study showed that Nanog might be helpful in sustaining the self-renewal and the undifferentiation of prostate cancer stem cells, and may serve as a marker for prostate cancer stem cells for isolation and identification.