The effect of mesenchymal stem cells on osteoclast precursor cell differentiation
The effect of mesenchymal stem cells on osteoclast precursor cell differentiation
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DOI：
10.2334/josnusd.17-0315
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发表时间：
2019-03-01
影响因子：
1.9
通讯作者：
Tanimoto, Kotaro
中科院分区：
文献类型：
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作者：
Abe, Takaharu;Sumi, Keisuke;Tanimoto, Kotaro
骨髓间充质干细胞（MSCs）的移植在再生医学领域得到了广泛的研究。骨再生是通过成骨细胞和破骨细胞的相互作用实现的。然而，MSC对破骨细胞的影响尚不清楚。本研究的目的是研究骨髓间充质干细胞与RAW 264细胞间接共培养后，使用qPCR对破骨细胞分化因子基因表达的影响。还比较了加入可溶性核因子κ B受体激活剂（NF-κ B）配体（sRANKL）后的破骨细胞数量。随着时间的推移，MSC的骨保护素（OPG）的表达在共培养中显著升高。加入sRANKL后RAW 264细胞向成熟破骨细胞的分化被与MSC共培养显著抑制。RAW 264细胞中RANK、集落刺激因子1受体、NF-κ B B和活化T细胞胞质核因子1的表达被与MSC共培养显著抑制。与RAW 264细胞共培养后，OPG蛋白的表达明显高于单独培养的MSCs，且明显高于RANKL的表达。MSCs通过表达OPG抑制破骨细胞分化。
Transplantation of mesenchymal stem cells (MSCs) has been extensively studied in the field of regenerative medicine. Bone regeneration is achieved via the interaction of osteoblasts and osteoclasts. However, the influence of MSCs on osteoclasts is unknown. The purpose of this study was to investigate the effect of MSCs on the expression of genes for osteoclast differentiation factors using qPCR after indirect co-culture of MSCs and RAW264 cells. The numbers of osteoclasts after addition of soluble receptor activator of nuclear factor kappa B (NF-kappa B) ligand (sRANKL) were also compared. Expression of osteoprotegerin (OPG) by MSCs was significantly elevated in co-culture over time. The differentiation of RAW264 cells into mature osteoclasts following addition of sRANKL was significantly inhibited by co-culture with MSCs. Expression of RANK, colony stimulating factor 1 receptor, NF-kappa B, and nuclear factor of activated T-cell cytoplasmic 1 in RAW264 cells was significantly inhibited by co-culture with MSCs. Expression of OPG protein was higher in co-culture with RAW264 cells than in MSCs alone, and the expression level was clearly higher than that of RANKL. MSCs appeared to inhibit osteoclast differentiation via expression of OPG.